Repeated use of a hydrophobic ligand-containing porous membrane for protein recovery
Repeated use of a hydrophobic ligand-containing porous membrane for protein recovery
复制标题
重复使用含有疏水配体的多孔膜进行蛋白质回收
DOI：
10.1016/s0376-7388(97)00143-9
复制
发表时间：
1997
影响因子：
9.5
通讯作者：
T. Sugo
中科院分区：
文献类型：
--
作者：
N. Kubota;Minoru Kounosu;Kyoichi Saito;K. Sugita;Kohei Watanabe;T. Sugo
以甲基丙烯酸缩水甘油酯为单体，通过辐射引发接枝聚合，然后与苯酚和水进行开环反应，制备了一种含有苯基疏水配体的多孔中空纤维膜。牛血清白蛋白（BSA）在含有2M（NH4）2SO4的磷酸盐缓冲液中的BSA溶液通过中空纤维的孔渗透过程中与配体结合。随后用不含（NH4）2SO4的缓冲液洗脱，洗脱百分比为82%。重复的吸附和洗脱循环导致BSA在孔表面上的积累，导致BSA的结合容量随着循环次数的增加而降低。相反，通过在每次洗脱后渗透1M NaOH，即使在10个循环后也保持BSA的结合能力。发现这种碱性再生在确保含苯基的多孔膜重复用于回收蛋白质方面是有效的。
A porous hollow-fiber membrane containing a phenyl group as a hydrophobic ligand was prepared by radiation-induced graft polymerization of glycidyl methacrylate, followed by successive ring-opening reactions with phenol and water. Bovine serum albumin (BSA) was bound to the ligand during permeation of a BSA solution in phosphate buffer containing 2M (NH4)2SO4through the pores of the hollow fiber. Subsequent elution with an (NH4)2SO4-free buffer exhibited an elution percentage of 82%. Repeated cycles of adsorption and elution caused the accumulation of BSA on the pore surface, resulting in a decrease in the binding capacity of BSA with increasing number of cycles. In contrast, by permeating 1 M NaOH after each elution, the binding capacity of BSA was maintained even after ten cycles. This alkaline regeneration was found to be effective in ensuring repeated use of the phenyl-group-containing porous membrane for recovery of proteins.