Characterization of jet injection efficiency with mouse cadavers
Characterization of jet injection efficiency with mouse cadavers
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小鼠尸体喷射注射效率的表征
DOI：
10.1016/j.jconrel.2019.05.023
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发表时间：
2019
影响因子：
10.8
通讯作者：
Lacerda, Carla M.R.
中科院分区：
文献类型：
--
作者：
Marston, Jeremy O.;Lacerda, Carla M.R.
无针药物递送因减少锐器浪费、预防针刺损伤以及改善药物分散和吸收的潜力而备受追捧。虽然有大量关于不同递送方法的文献，但喷射注射被提议作为递送粘性流体的唯一候选者，这与基于DNA的疫苗的出现特别相关。因此，本研究的重点是评估粘度和射流配置（即相对于皮肤的间距）对固定弹簧加载系统（Bioject ID Pen）注射效率的作用。我们在小鼠尸体的背景下进行了这项评估，发现确定成功率的主要因素是从安乐死开始的时间，这被认为是底层组织硬度的代表。对于总体注射效率，方差分析测试表明刚度非常显著（P< < 0.001），间距中度显著（P< 0.1），而粘度不显著。相比之下，当评价皮内递送的百分比时，发现粘度和间隙都是显著的（P< 0.01）。使用高分辨率微计算机断层扫描（μ-CT），我们还确定了注射后立即的深度和整体分散模式。
Needle-free drug delivery is highly sought after for reduction in sharps waste, prevention of needle-stick injuries, and potential for improved drug dispersion and uptake. Whilst there is a wealth of literature on the array of different delivery methods, jet injection is proposed as the sole candidate for delivery of viscous fluids, which is especially relevant with the advent of DNA-based vaccines. The focus of this study was therefore to assess the role of viscosity and jet configuration (i.e. stand-off relative to the skin) upon injection efficiency for a fixed spring-loaded system (Bioject ID Pen). We performed this assessment in the context of mouse cadavers and found that the dominant factor in determining success rates was the time from euthanasia, which was taken as a proxy for the stiffness of the underlying tissue. For overall injection efficiency, ANOVA tests indicated that stiffness was highly significant (P< < 0.001), stand-off was moderately significant (P< 0.1), and viscosity was insignificant. In contrast, both viscosity and standoff were found to be significant (P< 0.01) when evaluating the percentage delivered intradermally. Using high-resolution micro-computed tomography (μ-CT), we also determined the depth and overall dispersion pattern immediately after injection.