Multiscale osteointegration as a new paradigm for the design of calcium phosphate scaffolds for bone regeneration
Multiscale osteointegration as a new paradigm for the design of calcium phosphate scaffolds for bone regeneration
复制标题
DOI：
10.1016/j.biomaterials.2010.01.052
复制
发表时间：
2010-05-01
期刊：
影响因子：
14
通讯作者：
Johnson, Amy J. Wagoner
中科院分区：
文献类型：
--
作者：
Levengood, Sheeny K. Lan;Polak, Samantha J.;Johnson, Amy J. Wagoner
大孔尺寸(>；100微米)和几何形状在合成支架中骨再生中的作用已被广泛研究，但成功应用于临床的速度一直很慢。对微尺度(0.5-10微米)的孔隙度的关注明显较少。虽然一些研究表明，磷酸钙(CaP)支架中的微孔可以提高大孔中骨形成的速度和程度，但还没有人将微孔作为骨生长的额外和重要的空间来探索。在这里，我们展示了双相磷酸钙(BCP)支架在宏观和微观尺度上的骨整合。骨、类骨和成骨细胞填充支架杆中的微孔，骨细胞嵌入微孔中的矿化基质中，不添加生长因子。这项工作进一步强调了在微尺度上考虑设计参数的重要性，并展示了没有“死隙”的骨-支架复合材料的可能性。植入的骨细胞分布在微孔杆中，可能形成机械传感网络，这在没有微孔率的支架中是不可能的。多尺度骨整合通过长期和短期的力学性能、载荷传递和稳定性的改善，极大地提高了支架的整体性能，代表了支架设计的新范式。(C)2010爱思唯尔有限公司。保留所有权利。
The role of macropore size (>100 mu m) and geometry in synthetic scaffolds for bone regeneration has been studied extensively, but successful translation to the clinic has been slow. Significantly less attention has been given to porosity at the microscale (0.5-10 mu m). While some have shown that microporosity in calcium phosphate (CaP)-based scaffolds can improve rate and extent of bone formation in macropores, none has explored microporosity as an additional and important space for bone ingrowth. Here we show osteointegration of biphasic calcium phosphate (BCP) scaffolds at both the macro and micro length scales. Bone, osteoid, and osteogenic cells fill micropores in scaffold rods and osteocytes are embedded in mineralized matrix in micropores, without the addition of growth factors. This work further highlights the importance of considering design parameters at the microscale and demonstrates the possibility for a bone-scaffold composite with no "dead space." Embedded osteocytes distributed throughout microporous rods may form a mechanosensory network, which would not be possible in scaffolds without microporosity. Multiscale osteointegration has the potential to greatly improve overall performance of these scaffolds through an improvement of mechanical properties, load transfer, and stability in the long and short term, and represents a new paradigm for scaffold design. (C) 2010 Elsevier Ltd. All rights reserved.