Silk fibroin nanoscaffolds for neural tissue engineering
Silk fibroin nanoscaffolds for neural tissue engineering
复制标题
DOI：
10.1007/s10856-020-06422-5
复制
发表时间：
2020-08-28
影响因子：
3.7
通讯作者：
Clarkson, Andrew N.
中科院分区：
文献类型：
--
作者：
Boni, Rossana;Ali, Azam;Clarkson, Andrew N.
神经系统是身体的重要组成部分，对该系统的损害，无论是受伤还是疾病，都可能导致严重或潜在的致命后果。神经工程中的一个重要问题是，鉴于其复杂的生理学和有限的再生能力，我们如何刺激受损神经组织的再生。为了再生受损的神经组织，本研究从丝素蛋白（SF）、聚乙二醇（PEG）和聚乙烯醇（PVA）的生物材料混合物静电纺丝三维纳米支架（3DNSs）。对3DNS进行表征以确定其直接植入CNS的潜在适用性。使用PC12细胞在体外研究了3DNSs的生物活性，并使用小鼠缺血性中风的光血栓形成模型在体内评估了它们对反应性星形胶质细胞增生的影响。结果表明，SF的浓度直接影响3DNSs的力学特性和内部结构，其制剂呈现凝胶状结构（SF >= 50%）或纳米纤维状结构（SF
The nervous system is a crucial component of the body and damages to this system, either by injury or disease, can result in serious or potentially lethal consequences. An important problem in neural engineering is how we can stimulate the regeneration of damaged nervous tissue given its complex physiology and limited regenerative capacity. To regenerate damaged nervous tissue, this study electrospun three-dimensional nanoscaffolds (3DNSs) from a biomaterial blend of silk fibroin (SF), polyethylene glycol (PEG), and polyvinyl alcohol (PVA). The 3DNSs were characterised to ascertain their potential suitability for direct implant into the CNS. The biological activity of 3DNSs was investigated in vitro using PC12 cells and their effects on reactive astrogliosis were assessed in vivo using a photothrombotic model of ischaemic stroke in mice. Results showed that the concentration of SF directly affected the mechanical characteristics and internal structure of the 3DNSs, with formulations presenting as either a gel-like structure (SF >= 50%) or a nanofibrous structure (SF