FERULATE CROSS-LINKING IN CELL-WALLS ISOLATED FROM MAIZE CELL-SUSPENSIONS
FERULATE CROSS-LINKING IN CELL-WALLS ISOLATED FROM MAIZE CELL-SUSPENSIONS
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DOI：
10.1016/0031-9422(95)00413-2
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发表时间：
1995-11-01
期刊：
影响因子：
3.8
通讯作者：
AMRHEIN, N
中科院分区：
文献类型：
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作者：
GRABBER, JH;HATFIELD, RD;AMRHEIN, N
阿拉伯聚糖通过阿魏酸脱氢二聚体的交联和阿魏酰化阿拉伯聚糖掺入木质素的模型是用含有5.3-18.0 mg g（-1）总阿魏酸的玉米壁（Zea mays cv Black Mexican）进行的。总阿魏酸脱氢二聚体的比例增加约20至45%时，稀释过氧化氢加入到含有结合过氧化物酶的墙壁。约45%的脱氢二聚体通过8-5个键偶联，其中8-8、8-0-4和5-5个偶联的脱氢二聚体各自占总量的10-25%。当外源供给的羟基肉桂醇通过壁结合过氧化物酶和原位产生的过氧化氢聚合成壁时，通过皂化释放的阿魏酸的量减少83-95%。只有40%的阿魏酸纳入木质素的醚键水解后回收。这些结果表明，在草的初生壁成为广泛交联的阿魏酸和脱氢二阿魏酸在木质化过程中，只有一部分的阿魏酸在木质组织是可测量的，通过目前的溶剂分解方法。
Cross-linking of arabinoxylans by ferulate dehydrodimers and incorporation of feruloylated arabinoxylans into lignin were modelled with maize walls (Zea mays cv Black Mexican) containing 5.3-18.0 mg g(-1) of total ferulates. The proportion of dehydrodimers to total ferulates increased from ca 20 to 45% when dilute hydrogen peroxide was added to walls containing bound peroxidase. About 45% of the dehydrodimers were coupled by 8-5 linkages, with 8-8, 8-0-4 and 5-5 coupled dehydrodimers each comprising 10-25% of the total. The quantity of ferulates released by saponification were reduced by 83-95% when exogenously supplied hydroxycinnamyl alcohols were polymerized into walls by wall-bound peroxidase and in situ generated hydrogen peroxide. Only 40% of the ferulates incorporated into lignin were recovered following hydrolysis of ether linkages. These results indicate that primary walls in grasses become extensively cross-linked by ferulic and dehydrodiferulic acids during lignification, and that only a portion of ferulates in lignified tissues are measurable by current solvolytic methods.