Emerging heterogeneous compartments by viruses in single bacterial cells
Emerging heterogeneous compartments by viruses in single bacterial cells
复制标题
DOI：
10.1038/s41467-020-17515-8
复制
发表时间：
2020-07
影响因子：
16.6
通讯作者：
J. Trinh;Qiuyan Shao;J. Guan;Lanying Zeng
中科院分区：
文献类型：
--
作者：
J. Trinh;Qiuyan Shao;J. Guan;Lanying Zeng
生物过程的空间组织允许分子结果的可变性和协调发展。在这里，我们通过表征亚细胞空间中的病毒成分和过程来研究组织如何支持噬菌体 lambda 发育和决策。我们在单分子水平上使用活细胞和原位荧光成像来检查单细胞感染中的 lambda DNA 复制、转录、病毒粒子组装和资源招募，将感染周期的关键过程整合到涵盖空间和时间的噬菌体发育的连贯模型中。我们发现不同的病毒DNA在细胞内建立独立的亚细胞区室，从而维持单细胞中异质病毒的发育。这些单独的噬菌体区室由 E 物理分隔。类核苷。我们的结果提供了机制细节，描述了不同的病毒如何异质地发育以类似于单细胞表型。
Spatial organization of biological processes allows for variability in molecular outcomes and coordinated development. Here, we investigate how organization underpins phage lambda development and decision-making by characterizing viral components and processes in subcellular space. We use live-cell and in situ fluorescence imaging at the single-molecule level to examine lambda DNA replication, transcription, virion assembly, and resource recruitment in single-cell infections, uniting key processes of the infection cycle into a coherent model of phage development encompassing space and time. We find that different viral DNAs establish separate subcellular compartments within cells, which sustains heterogeneous viral development in single cells. These individual phage compartments are physically separated by theE. colinucleoid. Our results provide mechanistic details describing how separate viruses develop heterogeneously to resemble single-cell phenotypes.