Apparent elastic modulus and hysteresis of skeletal muscle cells throughout differentiation
Apparent elastic modulus and hysteresis of skeletal muscle cells throughout differentiation
复制标题
DOI：
10.1152/ajpcell.00502.2001
复制
发表时间：
2002-10-01
影响因子：
5.5
通讯作者：
Truskey, GA
中科院分区：
文献类型：
--
作者：
Collinsworth, AM;Zhang, S;Truskey, GA
用原子力显微镜观察了分化对哺乳动物心肌细胞横向力学性能的影响。表观弹性模量从未分化成肌细胞的11.5 ± 1.3 kPa增加到分化8天后的45.3 ± 4.0 kPa（P < 0.05）。粘度的相对贡献，从标准化滞后面积确定，范围为0.13 +/- 0.02至0.21 +/- 0.03，在整个分化过程中没有变化。肌球蛋白表达与表观弹性模量相关，但肌球蛋白和β-微管蛋白均与滞后相关。微管没有影响机械性能，因为处理与秋水仙碱没有改变表观弹性模量或滞后。用细胞松弛素D或2，3-丁二酮2-单肟处理导致表观弹性模量显著降低，但滞后没有变化。总之，骨骼肌细胞表现出粘弹性行为，在分化过程中发生变化，产生的横向弹性模量增加。在分化过程中的横向弹性模量的变化的主要贡献者是肌动蛋白和肌球蛋白。
The effect of differentiation on the transverse mechanical properties of mammalian myocytes was determined by using atomic force microscopy. The apparent elastic modulus increased from 11.5 +/- 1.3 kPa for undifferentiated myoblasts to 45.3 +/- 4.0 kPa after 8 days of differentiation (P < 0.05). The relative contribution of viscosity, as determined from the normalized hysteresis area, ranged from 0.13 +/- 0.02 to 0.21 +/- 0.03 and did not change throughout differentiation. Myosin expression correlated with the apparent elastic modulus, but neither myosin nor beta-tubulin were associated with hysteresis. Microtubules did not affect mechanical properties because treatment with colchicine did not alter the apparent elastic modulus or hysteresis. Treatment with cytochalasin D or 2,3-butanedione 2-monoxime led to a significant reduction in the apparent elastic modulus but no change in hysteresis. In summary, skeletal muscle cells exhibited viscoelastic behavior that changed during differentiation, yielding an increase in the transverse elastic modulus. Major contributors to changes in the transverse elastic modulus during differentiation were actin and myosin.