Levels of synovial fluid interleukin-1 receptor antagonist in rheumatoid arthritis and other arthropathies. Potential contribution from synovial fluid neutrophils.
Levels of synovial fluid interleukin-1 receptor antagonist in rheumatoid arthritis and other arthropathies. Potential contribution from synovial fluid neutrophils.
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类风湿性关节炎和其他关节病中滑液中白细胞介素 1 受体拮抗剂的水平。
DOI：
10.1002/art.1780360607
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发表时间：
1993
影响因子：
--
通讯作者：
Arend,WP
中科院分区：
文献类型：
--
作者：
Malyak,M;Swaney,RE;Arend,WP
目的：测定关节滑液（SF）中白细胞介素1受体拮抗剂（IL-1 ra）的水平，并确定SF中性粒细胞（PMN）的能力。方法：采用酶联免疫吸附法（ELISA）检测16例类风湿性关节炎（RA）患者外周血单个核细胞（PMN）分泌IL-1 ra的水平，并与正常对照组比较。关节炎（RA）（平均值17.1 ng/ml），18例感染性或炎性非RA关节病患者的6例样本（平均值10.6 ng/ml），11例非炎性SF样本均未检测到。SF IL-1 ra水平与SF PMN浓度呈正相关（r = 0.680，P < 0.00001）。分离的SF PMN在不存在信使RNA（mRNA）的情况下含有预先存在的IL-1 ra蛋白。此外，脂多糖和粒细胞-巨噬细胞集落刺激因子均诱导培养的SF PMN中IL-1 ra mRNA的适度增加。结论：IL-1 ra水平在>80%的RA SF样本中升高。SF PMN产生IL-1 ra，可能有助于SF中存在的IL-1 ra水平。
Objective.To measure synovial fluid (SF) levels of interleukin‐1 receptor antagonist (IL‐1ra) and to determine the capacity of SF neutrophils (PMN) to synthesize and release IL‐1ra.Methods.A sensitive and specific enzyme‐linked immunosorbent assay was used to measure SF IL‐1ra protein concentrations and IL‐1ra production by isolated SF PMN.Results.SF IL‐1ra levels were elevated in 13 of 16 samples from patients with rheumatoid arthritis (RA) (mean 17.1 ng/ml), in 6 of 18 samples from patients with infectious or inflammatory, non‐RA arthropathies (mean 10.6 ng/ml), and in none of 11 noninflammatory SF samples. SF IL‐1ra levels correlated with SF PMN concentrations (r = 0.680,P< 0.00001). Isolated SF PMN contained preexisting IL‐1ra protein in the absence of messenger RNA (mRNA). In addition, both lipopolysaccharide and granulocyte‐macrophage colonystimulating factor induced modest increases in IL‐1ra mRNA by cultured SF PMN.Conclusion.IL‐1ra levels are increased in >80% of RA SF samples. SF PMN produce IL‐1ra, possibly contributing to the levels of IL‐1ra present within the SF.