Biomarkers of oxidative stress and cellular‐based assays of indirect antioxidant measurement
Biomarkers of oxidative stress and cellular‐based assays of indirect antioxidant measurement
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DOI：
10.1002/9781119135388.ch9
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发表时间：
2017-12
期刊：
影响因子：
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通讯作者：
Cheng Yang;F. Shahidi;R. Tsao
中科院分区：
文献类型：
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作者：
Cheng Yang;F. Shahidi;R. Tsao
本章综述了自由基和氧化应激（OS）的细胞水平，以及由于氧化或氧化损伤不同生物分子而产生的OS生物标志物的形成，然后将这些生物标志物用于基于细胞的检测的最新研究。它还回顾了细胞内这种抗氧化作用背后的机制。OS生物标志物可分为三大类：诱导和产生OS的因素; OS的副产物;以及控制OS或减少氧化损伤的组分。OS的生物标志物可以使用间接测定方法检测。包括细胞抗氧化活性（CAA）在内的基于细胞的测定通过评估抗氧化酶或促氧化酶的表达或氧化还原转录因子的调节来测量OS生物标志物。本章然后讨论了间接抗氧化剂测量化合物通过监测氧化还原转录因子的激活和氧化酶或抗氧化酶的激活抑制。它进一步涵盖了基于细胞的测定系统中不同测量的优点和缺点。
This chapter reviews cellular levels of free radicals and oxidative stress (OS), and the formation of OS biomarkers produced as a result of oxidation or oxidative damage to different biomolecules, and then the latest research on using these biomarkers in cell‐based assay. It also reviews the mechanisms behind such antioxidant actions inside the cells. OS biomarkers can be classified into three main categories: factors that induce and generate OS; by‐products of OS; and components that control OS or reduce oxidative damage. Biomarkers of OS can be detected using indirect assay methods. Cellular‐based assays including cellular antioxidant activity (CAA) measure OS biomarkers by assessing the expression of antioxidant or prooxidant enzymes, or modulation of redox transcription factors. The chapter then discusses indirect antioxidant measurements of compounds through monitoring activation of redox transcription factors and inhibition of oxidases or activation of antioxidant enzymes. It further covers the advantages and disadvantages of different measurements in cellular‐based assay systems.