Quantification of CD8+ T lymphocytes responsive to human immunodeficiency virus (HIV) peptide antigens in HIV-infected patients and seronegative persons at high risk for recent HIV exposure
Quantification of CD8+ T lymphocytes responsive to human immunodeficiency virus (HIV) peptide antigens in HIV-infected patients and seronegative persons at high risk for recent HIV exposure
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DOI：
10.1086/517449
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发表时间：
1998-07-01
影响因子：
6.4
通讯作者：
Wölfel, T
中科院分区：
文献类型：
--
作者：
Herr, W;Protzer, U;Wölfel, T
结合肿瘤坏死因子(TNF)- α酶联免疫斑点（ELISPOT）检测和计算机辅助视频图像分析，检测和定量外周血CD8(+) T细胞与已知的人类免疫缺陷病毒1型（HIV-1）肽抗原（HLA-A2或HLA-A3）反应。在12名HIV-1感染患者中，有10名（83%）和11名没有I-W感染血清学和病毒学症状但近期性接触HIV-1风险高的患者中，有5名（45%）发现T淋巴细胞对至少一种HIV肽有反应。CD8(+) T细胞对HIV-1肽的反应在11名无HIV暴露史的HIV血清阴性供体中均未观察到。ELISPOT检测相对快速且易于执行，并且似乎可靠地检测由于先前暴露于HIV的T细胞反应性。这些发现支持使用ELISPOT检测来监测T细胞对HIV肽的反应性。
The combination of a tumor necrosis factor (TNF)-alpha enzyme-linked immunospot (ELISPOT) assay and computer-assisted video image analysis was used to detect and quantitate in peripheral blood CD8(+) T cells reactive with known human immunodeficiency virus type 1 (HIV-1) peptide antigens presented by HLA-A2 or HLA-A3. T lymphocyte responsiveness to at least one HIV peptide was found in 10 (83%) of 12 HIV-1-infected patients and in 5 (45%) of 11 persons who had no serologic and virologic signs of I-W infection but who were at high risk for recent sexual exposure to HIV-1. CD8(+) T cells responding to HIV-1 peptides were observed in none of 11 HIV-seronegative donors without a history of HIV exposure. ELISPOT assays are relatively fast and easy to perform and appear to reliably detect T cell reactivity due to previous exposure to HIV. These Findings support the use of the ELISPOT assay for monitoring T cell responsiveness to HIV peptides.