Effects of a wheat bran extract containing arabinoxylan oligosaccharides on gastrointestinal health parameters in healthy adult human volunteers: a double-blind, randomised, placebo-controlled, cross-over trial
Effects of a wheat bran extract containing arabinoxylan oligosaccharides on gastrointestinal health parameters in healthy adult human volunteers: a double-blind, randomised, placebo-controlled, cross-over trial
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DOI：
10.1017/s0007114512000372
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发表时间：
2012-12-28
影响因子：
3.6
通讯作者：
Broekaert, Willem F.
中科院分区：
文献类型：
--
作者：
Francois, Isabelle E. J. A.;Lescroart, Olivier;Broekaert, Willem F.
麦麸提取物（WBE）是一种食品级可溶性纤维制剂，高度富集阿拉伯木聚糖寡糖。在这项安慰剂对照的交叉人类干预试验中，研究了耐受性和对结肠蛋白质和碳水化合物发酵的影响。在1周的导入期后，63名健康成年志愿者以随机顺序服用3、10和0 g WBE/d，持续3周，每个治疗期之间有2周的洗脱期。在导入期结束时和每个治疗期结束时采集空腹血样，用于分析血液学和临床生化参数。此外，受试者收集粪便样本用于分析微生物群、SCFA和pH。收集超过48小时的尿液样本用于分析对甲酚和苯酚含量。最后，受试者完成问卷调查，对18种胃肠道症状的发生频率和痛苦程度进行评分。WBE摄入量为10 g/d后，尿中对甲酚排泄量显著降低。每日摄入10 g WBE后，粪便双歧杆菌水平显著增加。此外，10 g/d的WBE摄入量增加了粪便SCFA浓度并降低了粪便pH值，表明WBE向所需代谢物的结肠发酵增加。在10 g/d时，WBE引起肠胃气胀发生频率和痛苦严重程度的轻度增加，以及便秘发生频率轻度降低的趋势。总之，WBE在健康成人志愿者中的耐受性良好，剂量高达10 g/d。摄入10 g WBE/d对肠道健康参数产生有益影响。
Wheat bran extract (WBE) is a food-grade soluble fibre preparation that is highly enriched in arabinoxylan oligosaccharides. In this placebo-controlled cross-over human intervention trial, tolerance and effects on colonic protein and carbohydrate fermentation were studied. After a 1-week run-in period, sixty-three healthy adult volunteers consumed 3, 10 and 0 g WBE/d for 3 weeks in a random order, with 2 weeks' washout between each treatment period. Fasting blood samples were collected at the end of the run-in period and at the end of each treatment period for analysis of haematological and clinical chemistry parameters. Additionally, subjects collected a stool sample for analysis of microbiota, SCFA and pH. A urine sample, collected over 48 h, was used for analysis of p-cresol and phenol content. Finally, the subjects completed questionnaires scoring occurrence frequency and distress severity of eighteen gastrointestinal symptoms. Urinary p-cresol excretion was significantly decreased after WBE consumption at 10 g/d. Faecal bifidobacteria levels were significantly increased after daily intake of 10 g WBE. Additionally, WBE intake at 10 g/d increased faecal SCFA concentrations and lowered faecal pH, indicating increased colonic fermentation of WBE into desired metabolites. At 10 g/d, WBE caused a mild increase in flatulence occurrence frequency and distress severity and a tendency for a mild decrease in constipation occurrence frequency. In conclusion, WBE is well tolerated at doses up to 10 g/d in healthy adults volunteers. Intake of 10 g WBE/d exerts beneficial effects on gut health parameters.