Transmission ratio distortion in intraspecific hybrids of Mimulus guttatus:: Implications for genomic divergence
Transmission ratio distortion in intraspecific hybrids of Mimulus guttatus:: Implications for genomic divergence
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DOI：
10.1534/genetics.104.038653
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发表时间：
2005-05-01
期刊：
影响因子：
3.3
通讯作者：
Willis, JH
中科院分区：
文献类型：
--
作者：
Hall, MC;Willis, JH
我们构建了两个不同种群之间的遗传连锁图谱。我们利用 154 个 AFLP、微卫星和基于基因的标记对 F-2 作图群体 (N = 539) 进行了基因分型。构建了由 14 个连锁群上的 112 个标记位点组成的框架图谱，总图谱长度为 1518 cM Kosambi。近一半的标记 (48%) 表现出显着的传输比失真 (α = 0.05)。通过使用贝叶斯多点作图方法和对显着扭曲标记的目视检查，我们在整个基因组中检测到了 12 个传输比扭曲位点 (TRDL)。在该种内图谱中检测到的高度分离扭曲表明存在显着的基因组差异，这可能表明这两个群体之间的基因组不相容。我们将该图谱中的透射比失真模式与在 M. guttatus 和 M. nasutus 之间构建的种间图谱进行了比较。在两张图中都检测到了相似程度的偏析失真。比较图谱之间的共线区域，以确定酸浆属物种内部和之间是否存在非孟德尔分离扭曲的共享遗传模式。
We constructed a genetic linkage map between two divergent populations of Mimulus guttatus. We genotyped an F-2 mapping population (N = 539) at 154 AFLP, microsatellite, and gene-based markers. A framework map was constructed consisting of 112 marker loci on 14 linkage groups with a total map length of 1518 cM Kosambi. Nearly half of all markers (48%) exhibited significant transmission ratio distortion (alpha = 0.05). By using a Bayesian multipoint mapping method and visual inspection of significantly distorted markers, we detected 12 transmission ratio distorting loci (TRDL) throughout the genome. The high degree of segregation distortion detected in this intraspecific map indicates substantial genomic divergence that perhaps suggests genomic incompatibilities between these two populations. We compare the pattern of transmission ratio distortion in this map to an interspecific map constructed between M. guttatus and M. nasutus. A similar level of segregation distortion is detected in both maps. Collinear regions between maps are compared to determine if there are shared genetic patterns of non-Mendelian segregation distortion within and among Mimulus species.