Assessment of DNA damage and its modulation by dietary and genetic factors in smokers using the Comet assay: a biomarker model
Assessment of DNA damage and its modulation by dietary and genetic factors in smokers using the Comet assay: a biomarker model
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DOI：
10.1080/13547500500138963
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发表时间：
2005-03-01
期刊：
影响因子：
2.6
通讯作者：
Pool-Zobel, BL
中科院分区：
文献类型：
--
作者：
Glei, M;Habermann, N;Pool-Zobel, BL
需要评估人类暴露于遗传毒素的方法，并提高对饮食预防癌症的认识。彗星试验用于检测目标组织中与吸烟相关的暴露和饮食调节。在一项饮食干预研究中，使用补充益生元+/-抗氧化剂的面包，从38名健康男性志愿者（吸烟者和非吸烟者）中收集了口腔刮屑、血液和粪便。PCR法检测GSTM 1基因型。使用彗星试验分析颊和外周淋巴细胞的DNA损伤。在人结肠HT 29克隆19 A细胞中测定粪便水（FW）的遗传毒性。“尾强度”（TI）用作彗星试验中DNA损伤的定量指标。面包干预降低了吸烟者淋巴细胞的DNA损伤（8.3 +/-1.7%TI vs 10.2 +/-4.1%TI，n = 19），但未降低非吸烟者淋巴细胞的DNA损伤（8.6 +/-2.8%TI vs 8.3 +/-2.7%TI，n = 15）。粪便水遗传毒性仅在非吸烟者中降低（9.4 +/- 2.9% TI vs 18.9 +/- 13.1% TI，n = 15），但在吸烟者中未降低（15.5 +/- 10.7% TI vs 20.4 +/- 14.1% TI，n = 13）。彗星试验是有效的检测吸烟相关的暴露（口腔细胞）和饮食干预（粪便样本）的疗效。吸烟者和非吸烟者从益生元面包+/-抗氧化剂的干预中获益不同。按基因型分层的数据增强了干预效应的特异性/敏感性，并提供了易感性作用的重要信息。
Methods are needed to assess exposure to genotoxins in humans and to improve understanding of dietary cancer prevention. The Comet assay was used to detect smoking-related exposures and dietary modulations in target tissues. Buccal scrapings, blood and faeces were collected from 38 healthy male volunteers ( smokers and non-smokers) during a dietary intervention study with bread supplemented with prebiotics +/- antioxidants. GSTM1-genotype was determined with PCR. Buccal and peripheral lymphocytes were analysed for DNA damage using the Comet assay. Genotoxicity of faecal water (FW) was assayed in human colon HT29 clone 19A cells. 'Tail intensity' (TI) was used as a quantitative indicator of DNA damage in the Comet assay. Intervention with bread reduced DNA damage in lymphocytes of smokers (8.3 +/- 1.7% TI versus 10.2 +/- 4.1% TI, n = 19), but not of non-smokers (8.6 +/- 2.8% TI versus 8.3 +/- 2.7% TI, n = 15). Faecal water genotoxicity was reduced only in non-smokers (9.4 +/- 2.9% TI versus 18.9 +/- 13.1% TI, n = 15) but not in smokers (15.5 +/- 10.7% TI versus 20.4 +/- 14.1% TI, n = 13). The Comet assay was efficient in the detection of both smoking-related exposure ( buccal cells) and efficacy of dietary intervention ( faecal samples). Smokers and non-smokers profited differently from the intervention with prebiotic bread +/- antioxidants. Stratification of data by genotype enhanced specificity/sensitivity of the intervention effects and contributed important information on the role of susceptibility.