Impaired developmental competence of oocytes in adult prenatally androgenized female rhesus monkeys undergoing gonadotropin stimulation for in vitro fertilization.
Impaired developmental competence of oocytes in adult prenatally androgenized female rhesus monkeys undergoing gonadotropin stimulation for in vitro fertilization.
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DOI：
10.1210/jcem.87.3.8287
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发表时间：
2002-03
期刊：
影响因子：
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通讯作者：
D. Dumesic;R. D. Schramm;Eric T. Peterson;A. Paprocki;Rao Zhou;David H. Abbott
中科院分区：
文献类型：
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作者：
D. Dumesic;R. D. Schramm;Eric T. Peterson;A. Paprocki;Rao Zhou;David H. Abbott
为了确定孕d 40-44(早期治疗)或100-115(晚期治疗)开始的孕期丙酸暴露是否影响卵母细胞的功能，对5只孕前雄激素化和5只晚期治疗的正常猕猴进行了hCG注射后取卵的重组人FSH注射。基础、促性腺激素刺激和取卵时测定血清FSH、LH、雌二醇(E(2))、孕酮(P(4))、雄烯二酮(A(4))、睾酮(T)和双氢睾酮；基础和取卵时测定空腹血糖和胰岛素。对卵泡液中性激素进行分析。卵子数量、核成熟度和受精率在雌性组之间没有差异，但早期处理的产前雄激素化的雌性受精卵发育成囊胚的比例降低。卵泡内P(4)/E(2)比值在早期治疗前雄激素化女性显著升高，而卵泡内P(4)/A(4)和T/A(4)比值在所有产前雄激素化女性均显著升高。早期治疗的胎儿期雄激素升高的女性表现出持续性的黄体生成素高分泌。在促性腺激素刺激期间，他们也无法抑制循环中的胰岛素水平。所有女性的循环性类固醇水平和血清P(4)/E(2)、P(4)/A(4)和E(2)/雄激素比值相似。在接受促性腺激素治疗的猴子中，早期产前雄激素治疗会损害卵母细胞的发育能力，并在促黄体生成素高分泌和相对胰岛素过剩的情况下诱导卵泡提前分化。
To determine whether prenatal T propionate exposure beginning gestational d 40-44 (early-treated) or 100-115 (late-treated) affects oocyte competence, five early-treated and five late-treated prenatally androgenized and five normal monkeys underwent recombinant human FSH injections with oocyte-retrieval after hCG administration. Serum FSH, LH, estradiol (E(2)), progesterone (P(4)), androstenedione (A(4)), T, and dihydrotestosterone were measured basally, during gonadotropin stimulation and at oocyte-retrieval; fasting serum glucose and insulin also were determined basally and at oocyte-retrieval. Follicle fluid sex steroids were analyzed. Oocyte number, nuclear maturity, and fertilization were comparable among female groups, but the percentage of zygotes developing into blastocysts was reduced in early-treated prenatally androgenized females. The intrafollicular P(4)/E(2) ratio was significantly elevated in early-treated prenatally androgenized females, whereas intrafollicular P(4)/A(4) and T/A(4) ratios were significantly increased in all prenatally androgenized females. Early-treated prenatally androgenized females demonstrated persistent LH hypersecretion. They also were unable to suppress circulating insulin levels during gonadotropin stimulation. Circulating sex steroid levels and serum P(4)/E(2), P(4)/A(4), and E(2)/androgen ratios were similar in all females. Early prenatal androgenization in monkeys receiving gonadotropins impairs oocyte developmental competence and seems to induce premature follicle differentiation in the presence of LH hypersecretion and relative insulin excess.