Temporal profile of Src, SSeCKS, and angiogenic factors after focal cerebral ischemia: correlations with angiogenesis and cerebral edema.
Temporal profile of Src, SSeCKS, and angiogenic factors after focal cerebral ischemia: correlations with angiogenesis and cerebral edema.
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DOI：
10.1016/j.neuint.2011.02.014
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发表时间：
2011-07
影响因子：
4.2
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Zan L;Wu H;Jiang J;Zhao S;Song Y;Teng G;Li H;Jia Y;Zhou M;Zhang X;Qi J;Wang J
更好地了解血管生成和血管通透性的潜在机制对于制定缺血性损伤的治疗策略是必要的。本研究的目的是检查大脑中动脉闭塞（MCAO）后大脑中Src和Src抑制的C激酶底物（SSeCKS）的空间和时间表达，并阐明Src、SSeCKS和中风后存在的关键血管生成因子之间的关系。对大鼠进行 MCAO 或假手术。逆转录聚合酶链反应和蛋白质印迹结果显示，Src早在MCAO后2小时就开始逐渐升高，并保持高水平1天。相反，MCAO 后 SSeCKS 下降。 Src表达与血管内皮生长因子和血管生成素-2呈正相关，与SSeCKS、血管生成素-1和闭锁小带-1呈负相关。然而，SSeCKS 具有相反的相关性。这些因子表达的变化与血管生成和脑水肿的进展相关。 Src 和 SSeCKS 表达的动态时间变化可能调节局灶性脑缺血后的血管生成和脑水肿形成。
A better understanding of the underlying mechanisms of angiogenesis and vascular permeability is necessary for the development of therapeutic strategies for ischemic injury. The purpose of this study was to examine the spatial and temporal expression of Src and Src-suppressed C Kinase Substrate (SSeCKS) in brain after middle cerebral artery occlusion (MCAO) and elucidate the relationships among Src, SSeCKS, and the key angiogenic factors present after stroke. Rats were subjected to either MCAO or sham operation. Reverse transcriptase-polymerase chain reaction and Western blotting results revealed that Src gradually increased starting as early as 2 h after MCAO and remained high for 1 day. In contrast, SSeCKS decreased after MCAO. Src expression correlated positively with that of vascular endothelial growth factor and angiopoietin-2, and negatively with that of SSeCKS, angiopoietin-1, and zonula occludens-1. However, SSeCKS had the reverse correlations. Changes in the expression of these factors correlated with the progress of angiogenesis and cerebral edema. Dynamic temporal changes in Src and SSeCKS expression may modulate angiogenesis and cerebral edema formation after focal cerebral ischemia.