Petunia floral volatile benzenoid/phenylpropanoid genes are regulated in a similar manner
Petunia floral volatile benzenoid/phenylpropanoid genes are regulated in a similar manner
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DOI：
10.1016/j.phytochem.2009.09.036
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发表时间：
2010-02-01
期刊：
影响因子：
3.8
通讯作者：
Clark, David G.
中科院分区：
文献类型：
--
作者：
Colquhoun, Thomas A.;Verdonk, Julian C.;Clark, David G.
矮牵牛（矮牵牛×杂交种‘米切尔二倍体’[MD]）花从开花到衰老以协调一致的方式释放高水平的多种花挥发性苯类/苯丙酸（FVBP）化合物。在这里，我们显示七个基因负责生产的发射FVBP共享类似的转录积累档案，通过分析的四个表达标准。作为一个群体，FVBP基因转录本从开花到衰老在MD花的花瓣肢组织中积累到高水平。2 - 4小时的外源乙烯暴露会降低所有FVBP基因的转录水平，但2小时的处理不会加速衰老或减少MD花的挥发性排放。FVBP基因表现出两种明显的转录本积累的节律模式，然而，FVBP基因产物的一个子集的相应的酶活性不。总之，这些结果描绘花挥发性苯类/苯丙素类生物合成作为一个特定的系统与多个监管功能。一个这样的特征是FVBP基因的高度调节的转录物积累。此外，乙烯可能在花衰老程序之前在FVBP系统中具有调节作用。出版社：Elsevier Ltd
Petunia (Petunia x hybrida cv 'Mitchell Diploid' [MD]) flowers emit high levels of multiple floral volatile benzenoid/phenylpropanoid (FVBP) compounds from anthesis to senescence in a concerted manner. Here we show seven genes responsible for the production of emitted FVBPs share similar transcript accumulation profiles through an analysis of four expression criteria. As a group, the FVBP gene transcripts accumulate to high levels in petal limb tissue of MD flowers from anthesis to senescence. Two to four hours of exogenous ethylene exposure reduces transcript levels of all FVBP genes examined, but 2 h of treatment will not accelerate senescence or reduce volatile emissions in MD flowers. The FVBP genes show two obvious rhythmic patterns of transcript accumulation; however, corresponding enzyme activities of a subset of FVBP gene products do not. Together, these results depict floral volatile benzenoid/phenylpropanoid biosynthesis as a specific system with multiple regulatory features. One such feature is the highly regulated transcript accumulation of the FVBP genes. Additionally, ethylene may have a regulatory role in the FVBP system prior to a floral senescence program. Published by Elsevier Ltd.