Elevated temperature increases carbon and nitrogen fluxes between phytoplankton and heterotrophic bacteria through physical attachment
Elevated temperature increases carbon and nitrogen fluxes between phytoplankton and heterotrophic bacteria through physical attachment
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DOI：
10.1038/ismej.2016.156
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发表时间：
2017-03-01
期刊：
影响因子：
11
通讯作者：
Mayali, Xavier
中科院分区：
文献类型：
--
作者：
Arandia-Gorostidi, Nestor;Weber, Peter K.;Mayali, Xavier
量化海洋微生物对碳和氮循环的贡献及其对预测的海洋变暖的响应是微生物海洋学的主要挑战之一。在这里，我们提出了一个单细胞NanoSIMS同位素分析，以量化自由生活和附着的浮游植物和异养细菌的C和N吸收，以及它们对短期实验升温4摄氏度的反应。升高的温度增加总固碳超过50%，其中一个小的，但显着的分数被转移到异养在12小时内。细胞间附着使异养细菌的次级C吸收增加一倍，并使自养生物的次级N掺入增加68%。变暖还增加了80%的浮游植物与附着异养生物的丰度，并促进C从浮游植物转移到细菌的17%和N从细菌转移到浮游植物的50%。我们的研究结果表明，phytophiloton-bacteria附件提供了一个生态优势，营养掺入，这表明一个互惠互利的关系，似乎是由温度升高增强。
Quantifying the contribution of marine microorganisms to carbon and nitrogen cycles and their response to predicted ocean warming is one of the main challenges of microbial oceanography. Here we present a single-cell NanoSIMS isotope analysis to quantify C and N uptake by free-living and attached phytoplankton and heterotrophic bacteria, and their response to short-term experimental warming of 4 degrees C. Elevated temperature increased total C fixation by over 50%, a small but significant fraction of which was transferred to heterotrophs within 12 h. Cell-to-cell attachment doubled the secondary C uptake by heterotrophic bacteria and increased secondary N incorporation by autotrophs by 68%. Warming also increased the abundance of phytoplankton with attached heterotrophs by 80%, and promoted C transfer from phytoplankton to bacteria by 17% and N transfer from bacteria to phytoplankton by 50%. Our results indicate that phytoplankton-bacteria attachment provides an ecological advantage for nutrient incorporation, suggesting a mutualistic relationship that appears to be enhanced by temperature increases.