Micro-computed tomography for evaluating alveolar bone resorption induced by hyperocclusion
Micro-computed tomography for evaluating alveolar bone resorption induced by hyperocclusion
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DOI：
10.1016/j.jpor.2017.11.004
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发表时间：
2018-07-01
影响因子：
3.6
通讯作者：
Takahashi，Yutaka
中科院分区：
文献类型：
--
作者：
Tsutsumi，Takashi;Kajiya，Hiroshi;Takahashi，Yutaka
目的：咬合创伤是在过度咬合状态下，由于机械应力过大而导致牙槽骨破坏的一种牙周病。先前，我们已经证明C-C趋化因子配体(CCL)2/CCR2受体轴在MS依赖的破骨细胞形成中起关键作用。然而，在以前的工作中，我们无法精确测量牙槽骨的变化。在本研究中，我们试图建立一种准确的方法来评估过度咬合引起的牙槽骨吸收。方法：在麻醉下，在5周龄的C57/BL6野生型(WT)小鼠、CCL2-/-小鼠和CCR2-/-小鼠的磨牙上连接一根不锈钢丝，以诱导咬合力超负荷。结果：WT小鼠第7天牙槽骨骨密度较0天明显降低，可见明显的牙槽骨吸收现象。同样，CCL2-/-和CCR2-/-小鼠在第7天与第0天相比有明显的牙槽骨吸收。结论：显微CT图像可用于测量过度咬合模型小鼠骨密度的变化。该方法可用于进一步研究其他牙周病研究领域的骨骼变化。
Purpose: Occlusal trauma, resulting in the destruction of alveolar bone, is a form of periodontal disease caused by excessive mechanical stress (MS) during hyperocclusion. Previously, we showed that C--C chemokine ligand (CCL) 2/CCR2 receptor axis plays a crucial role in MS-dependent osteoclastogenesis. However, in the previous work, we were unable to precisely measure changes in alveolar bone profiles. In the present study, we sought to establish a precise method for evaluating alveolar bone resorption induced by hyperocclusion using micro-computed tomography.Methods: Under anesthesia, a stainless steel wire was attached to the molars of 5-week-old C57/BL6 wildtype (WT) mice, CCL2-/-mice, and CCR2-/-mice to induce occlusal force overload. At days 0 and 7, hard tissue samples were harvested and analyzed by micro-computed tomography.Results: In the WT mice, bone mineral density of the alveolar bone was significantly decreased at day 7 as compared with day 0, with marked alveolar bone resorption observed. Similarly, significant alveolar bone resorption was observed in the CCL2-/-and CCR2-/-mice at day 7 as compared with day 0.Conclusions: Micro-computed tomographic images can be used to measure changes in bone mineral density in a mouse model of hyperocclusion. This method may be useful for further investigating bone changes in other periodontal disease research fields.