Use of molecular beacons for the rapid analysis of DNA damage induced by exposure to an atmospheric pressure plasma jet
Use of molecular beacons for the rapid analysis of DNA damage induced by exposure to an atmospheric pressure plasma jet
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DOI：
10.1063/1.4939044
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发表时间：
2015-12
影响因子：
4
通讯作者：
H. Kurita;Saki Miyachika;H. Yasuda;K. Takashima;A. Mizuno
中科院分区：
文献类型：
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作者：
H. Kurita;Saki Miyachika;H. Yasuda;K. Takashima;A. Mizuno
一种快速的方法，用于评估暴露于等离子体后对DNA分子造成的损伤。在这里，我们建议使用的分子信标的快速检测大气压等离子体射流（APPJ）照射引起的DNA链断裂。通过APPJ照射切断分子信标导致荧光团-猝灭剂对分离，导致与DNA链断裂直接相关的荧光增加。结果表明，荧光强度的增加与曝光时间成正比，荧光增加率与放电功率成正比。这种简单而快速的方法允许估计暴露于非热等离子体引起的DNA损伤。
A rapid method for evaluating the damage caused to DNA molecules upon exposure to plasma is demonstrated. Here, we propose the use of a molecular beacon for rapid detection of DNA strand breaks induced by atmospheric pressure plasma jet (APPJ) irradiation. Scission of the molecular beacon by APPJ irradiation leads to separation of the fluorophore-quencher pair, resulting in an increase in fluorescence that directly correlates with the DNA strand breaks. The results show that the increase in fluorescence intensity is proportional to the exposure time and the rate of fluorescence increase is proportional to the discharge power. This simple and rapid method allows the estimation of DNA damage induced by exposure to a non-thermal plasma.