Dynamic behavior of microbial populations in stirred bioreactors simulated with Euler-Lagrange methods:: Traveling along the lifelines of single cells
Dynamic behavior of microbial populations in stirred bioreactors simulated with Euler-Lagrange methods:: Traveling along the lifelines of single cells
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DOI：
10.1021/ie030786k
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发表时间：
2004-08-04
影响因子：
4.2
通讯作者：
Reuss, M
中科院分区：
文献类型：
--
作者：
Lapin, A;Müller, D;Reuss, M
在目前的工作中，Euler-Lagrange方法已被应用到表征的行为的异质细胞群体在搅拌槽生物反应器与非理想混合。它允许人们将群体行为描述为单个细胞的胞内状态与反应器中的湍流场之间相互作用的结果。所采用的建模方法和数值方法是基于欧拉-拉格朗日制定的系统结合分数步方法，以允许一个稳定的，准确的，和数值有效的解决方案的基本方程。这种策略允许一个帐户的异质性存在于真实的反应器中的流体和细胞相，分别。通过三个实例说明了该方法的应用。建议的方法是很容易转移到类似的问题，需要一个隔离和高维的描述的内部结构的微粒相，这通常排除了人口平衡方程的应用。
In the present work, an Euler-Lagrange approach has been applied to characterize the behavior of a heterogeneous cell population in a stirred-tank bioreactor with nonideal mixing. It allows one to describe population behavior as the outcome of the interaction between the intracellular state of its individual cells and the turbulent flow field in the reactor. The modeling approach and the numerical method employed are based on an Euler-Lagrange formulation of the system combined with a fractional-step method to allow for a stable, accurate, and numerically efficient solution of the underlying equations. This strategy permits one to account for the heterogeneity present in real reactors in both the fluid and cellular phases, respectively. Three examples are used to illustrate the application of this approach. The suggested method is easily transferable to similar problems requiring a segregated and high-dimensional description of the internal structure of the corpuscular phase, which usually precludes the application of population balance equations.