Imaging the Microprocesses in Biofilm Matrices
Imaging the Microprocesses in Biofilm Matrices
复制标题
生物膜基质中的微过程成像
DOI：
10.1016/j.tibtech.2018.07.006
复制
发表时间：
2019
影响因子：
17.3
通讯作者：
Feng Bo
中科院分区：
文献类型：
--
作者：
Zhang Peng;Chen Youpeng;Qiu Juhui;Dai Youzhi;Feng Bo
生物膜是微生物和细胞外基质的聚集体，广泛存在于自然水体、污水处理系统和人体组织中，在水净化、生物污染和传染病中发挥着重要作用。近年来，人们发展了多种成像手段来显示生物膜内的形态结构和物质分布，并探索生物膜基质中的微过程，包括生物膜的形成、底物的转移和代谢以及细胞与细胞之间的通讯。这些技术提高了我们对生物膜控制和生物膜底物命运的理解。在这篇综述中，我们描述了各种成像技术的原理，并讨论了每种方法在表征生物膜基质中微过程的优势和局限性。
Biofilms, which are aggregates of microorganisms and extracellular matrices, widely colonize natural water bodies, wastewater treatment systems, and body tissues, and have vital roles in water purification, biofouling, and infectious diseases. Recently, multiple imaging modalities have been developed to visualize the morphological structure and material distribution within biofilms and to probe the microprocesses in biofilm matrices, including biofilm formation, transfer and metabolism of substrates, and cell–cell communication. These technologies have improved our understanding of biofilm control and the fates of substrates in biofilms. In this review, we describe the principles of various imaging techniques and discuss the advantages and limitations of each approach to characterizing microprocesses in biofilm matrices.