Potential role of Th17 cells in the pathogenesis of adult-onset Still's disease
Potential role of Th17 cells in the pathogenesis of adult-onset Still's disease
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DOI：
10.1093/rheumatology/keq284
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发表时间：
2010-12-01
期刊：
影响因子：
5.5
通讯作者：
Hsieh, Chia-Wei
中科院分区：
文献类型：
--
作者：
Chen, Der-Yuan;Chen, Yi-Ming;Hsieh, Chia-Wei
方法.采用细胞内细胞因子染色和流式细胞术测定了24名未经治疗的活动性AOSD患者、16名活动性SLE患者和12名健康志愿者循环Th 17细胞的频率。ELISA法检测血清IL-1 β、IL-6、IL-17、IL-18、IL-21和IL-23水平。活动期未治疗的AOSD患者（1.01%）和活动期SLE患者（1.26%）外周血Th 17细胞的中位频率明显高于健康志愿者（0.12%）（P均< 0.001）。AOSD患者外周血Th 17细胞频率与活动性评分（r = 0.527，P < 0.01）和血清铁蛋白水平（r = 0.724，P < 0.001）呈正相关，SLE患者外周血Th 17细胞频率与SLEDAI呈正相关（r = 0.663，P < 0.01）。此外，在AOSD和SLE患者中，循环Th 17细胞的频率与血清IL-1 β、IL-6、IL-17、IL-18、IL-21和IL-23水平呈正相关且显著。AOSD患者经有效治疗后，外周血Th 17细胞比例和血清IL-17水平均明显下降（P均< 0.001）。在我们的AOSD患者中，循环Th 17细胞的频率升高以及与疾病活动性的正相关性表明，Th 17细胞有助于这种疾病的发病机制。Th 17细胞的失调可能是AOSD和SLE发展的共同致病机制。
Methods. The frequencies of circulating Th17 cells in 24 patients with active untreated AOSD, 16 patients with active SLE and 12 healthy volunteers were determined using intracellular cytokine staining and flow cytometry. Serum levels of Th17-related cytokines, including IL-1 beta, IL-6, IL-17, IL-18, IL-21 and IL-23 were measured by ELISA.Results. Significantly higher median frequencies of circulating Th17 cells were found in active untreated AOSD patients (1.01%) and active SLE patients (1.26%) than in healthy volunteers (0.12%, both P < 0.001). The frequencies of circulating Th17 cells were positively correlated with activity score (r = 0.527, P < 0.01) and serum ferritin levels (r = 0.724, P < 0.001) in AOSD patients, and correlated with SLEDAI (r = 0.663, P < 0.01) in SLE patients. Additionally, the frequencies of circulating Th17 cells were positively and significantly correlated with serum levels of IL-1 beta, IL-6, IL-17, IL-18, IL-21 and IL-23 in both AOSD and SLE patients. The frequencies of circulating Th17 cells and serum IL-17 levels significantly decreased after effective therapy in AOSD patients (both P < 0.001).Conclusion. Elevated frequencies of circulating Th17 cells and a positive correlation with disease activity in our AOSD patients suggest that Th17 cells contribute to the pathogenesis of this disease. Dysregulation of Th17 cells may be a common pathogenic mechanism that underlies the development of both AOSD and SLE.