Infrageneric phylogeny of the genus Gentiana (Gentianaceae) inferred from nucleotide sequences of the internal transcribed spacers (ITS) of nuclear ribosomal DNA
Infrageneric phylogeny of the genus Gentiana (Gentianaceae) inferred from nucleotide sequences of the internal transcribed spacers (ITS) of nuclear ribosomal DNA
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DOI：
10.1002/j.1537-2197.1996.tb12750.x
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发表时间：
1996-05
影响因子：
3
通讯作者：
Yong-Ming Yuan;P. Küpfer;J. Doyle
中科院分区：
文献类型：
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作者：
Yong-Ming Yuan;P. Küpfer;J. Doyle
已对 20 个龙胆属物种的核糖体 DNA 内转录间隔区 (ITS) 进行了测序。通过整合先前发布的 8 个物种的序列数据，使用 Fitch 简约性和特征状态加权简约性进行了系统发育分析。所调查的类群中 ITS 1 的长度范围为 223 至 238 bp，ITS2 的长度范围为 216 至 234 bp。 ITS1 中物种对之间的序列差异为 5.0% 至 48.9%，ITS2 中为 1.1% 至 45.3%，ITS1 和 ITS2 组合数据中为 3.2% 至 46.1%。 ITS 系统发育总体上与形态学分类一致，除了 G.研究表明，马蹄草科与龙胆科密切相关，而不是肺炎科，而马艽科则被证明是龙胆属的一个并系群，最好将其从该属中排除。三个欧洲特有科和该属除隐性科以外的其余科之间存在差异。因此，该属的欧洲物种在一起并不形成单系群。软骨藻科之间关系密切。湖建议使用（包括Dolichocarpa节）、Cruciata和Pneumonanthe。冷冻科。湖（包括Monopodiae、Isomeria、Microsperma 和Phylllocalyx 部分）包含两个得到充分支持的进化枝：Frigidaes 部分。海峡和所有其他人一起。典型的非倍体组部分软骨藻的单系。湖被证实。 ITS 系统发育上染色体数目的优化表明，2/1 = 26 是包含 Frigidaes 组的进化枝的拟态状态。 l.、Cruciata、Pneumonanthe 和 Chondrophyllaes。 l.，并且可能 2n= 20 是异倍体组（Chondrophyllaes 组）的拟形状态。湖
The internal transcribed spacers (ITSs) of nuclear ribosomal DNA have been sequenced for 20 species ofGentiana.By incorporating previously released sequence data of eight species, phylogenelic analyses using Fitch parsimony and character‐state weighted parsimony were carried out. The length of ITS 1 in the taxa surveyed ranged from 223 to 238 bp and ITS2 from 216 to 234 bp. Sequence divergence between pairs of species ranged from 5.0% to 48.9% in ITS1, from 1.1% to 45.3% in ITS2, and from 3.2% to 46.1% in combined data of ITS1 and ITS2. The ITS phylogeny was generally congruent with morphological classifications except thatG. asclepiadeawas revealed to be closely related to sectionGentianainstead of sectionPneumonantheand sectionStenogynewas shown to be a paraphyletic group of the genusGentianathat would be better excluded from the genus. A divergence among the three European endemic sections and the remaining sections of the genus other than sectionStenogynewas revealed. Thus the European species of the genus together do not form a monophyletic group. A close relationship between the sectionsChondrophyllaes. l. (including sectionDolichocarpa),CruciataandPneumonanthewas suggested. The sectionFrigidaes. l. (including sectionsMonopodiae, Isomeria, Microsperma, andPhyllocalyx) contained two well‐supported clades: sectionFrigidaes. str. and all others together. The monophyly of the typically dysploid group sectionChondrophyllaes. l. was confirmed. Optimization of chromosome numbers on the ITS phylogeny suggested that 2/1 = 26 is a plesiomorphic state for the clade comprising sectionsFrigidaes. l.,Cruciata, Pneumonanthe, andChondrophyllaes. l., and probably 2n= 20 is a plesiomorphic state for the dysploid group, sectionChondrophyllaes. l.