Layer-specific optogenetic activation of pyramidal neurons causes beta-gamma entrainment of neonatal networks.
Layer-specific optogenetic activation of pyramidal neurons causes beta-gamma entrainment of neonatal networks.
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DOI：
10.1038/ncomms14563
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发表时间：
2017-02-20
影响因子：
16.6
通讯作者：
Hanganu-Opatz IL
中科院分区：
文献类型：
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作者：
Bitzenhofer SH;Ahlbeck J;Wolff A;Wiegert JS;Gee CE;Oertner TG;Hanganu-Opatz IL
大脑发育中的协调活动模式可能有助于神经回路的连接，这是未来行为需求的基础。然而，由于缺乏在早期发育过程中选择性操纵振荡的工具，很难获得这一假设的因果证据。我们建立了一种结合光遗传学和新生小鼠体内电生理记录的方案，以阐明前额皮质早期网络振荡的底物。我们发现，在子宫内通过高效通道视紫红质电穿孔转染的II/III层锥体神经元的光诱导激活驱动频率特异性尖峰，并促进β - γ频率范围内的网络振荡。相比之下，V/VI层锥体神经元的激活导致非特异性网络激活。因此，新生儿前额叶网络在快节奏中的牵引依赖于II/III层锥体神经元的激活。这里使用的这种方法可能对进一步询问正在发育的电路及其行为读数有用。发育过程中皮层活动的振荡对功能成熟很重要。在这里，作者在新生小鼠中使用光遗传学来确定不同前边缘皮质层锥体细胞放电在产生β - γ范围活动中的因果作用。
Coordinated activity patterns in the developing brain may contribute to the wiring of neuronal circuits underlying future behavioural requirements. However, causal evidence for this hypothesis has been difficult to obtain owing to the absence of tools for selective manipulation of oscillations during early development. We established a protocol that combines optogenetics with electrophysiological recordings from neonatal mice in vivo to elucidate the substrate of early network oscillations in the prefrontal cortex. We show that light-induced activation of layer II/III pyramidal neurons that are transfected by in utero electroporation with a high-efficiency channelrhodopsin drives frequency-specific spiking and boosts network oscillations within beta–gamma frequency range. By contrast, activation of layer V/VI pyramidal neurons causes nonspecific network activation. Thus, entrainment of neonatal prefrontal networks in fast rhythms relies on the activation of layer II/III pyramidal neurons. This approach used here may be useful for further interrogation of developing circuits, and their behavioural readout. Oscillations in cortical activity during development are important for functional maturation. Here, the authors use optogenetics in neonatal mice to determine a causal role for pyramidal cell firing in different prelimbic cortex layers in generating beta–gamma range activity.