B cells Can Modulate the CD8 Memory T Cell after DNA Vaccination Against Experimental Tuberculosis.
B cells Can Modulate the CD8 Memory T Cell after DNA Vaccination Against Experimental Tuberculosis.
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DOI：
10.1186/1479-0556-9-5
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发表时间：
2011-03-14
期刊：
影响因子：
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通讯作者：
Coelho-Castelo AA
中科院分区：
文献类型：
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作者：
Almeida LP;Trombone AP;Lorenzi JC;Rocha CD;Malardo T;Fontoura IC;Gembre AF;Silva RL;Silva CL;Castelo AP;Coelho-Castelo AA
尽管B细胞在免疫应答期间作为抗原呈递细胞（APC）是重要的，但它们在DNA疫苗接种模型中的作用是未知的。本研究通过体外和体内实验来评价B细胞保护小鼠抵抗结核分枝杆菌攻击的能力。体内外研究表明，B细胞能有效地呈递M.麻风65-kDa热休克蛋白（pcDNA 3-Hsp 65）内化和保护B基因敲除（BKO）小鼠免受结核分枝杆菌感染与野生型小鼠相比，pcDNA 3-Hsp 65转染的B细胞过继转移到BKO小鼠中挽救了记忆表型并减少了CFU的数量。这些数据不仅表明B细胞在诱导CD 8 T细胞中起重要作用，而且还表明它们在DNA疫苗模型中改善细菌清除。
Although B cells are important as antigen presenting cells (APC) during the immune response, their role in DNA vaccination models is unknown. In this study in vitro and in vivo experiments were performed to evaluate the ability of B cells to protect mice against Mycobacterium tuberculosis challenge. In vitro and in vivo studies showed that B cells efficiently present antigens after naked plasmid pcDNA3 encoding M. leprae 65-kDa heat shock protein (pcDNA3-Hsp65) internalization and protect B knock-out (BKO) mice against Mycobacterium tuberculosis infection. pcDNA3-Hsp65-transfected B cells adoptively transferred into BKO mice rescued the memory phenotypes and reduced the number of CFU compared to wild-type mice. These data not only suggest that B cells play an important role in the induction of CD8 T cells but also that they improve bacterial clearance in DNA vaccine model.