Expression of vascular endothelial growth factor in normal liver and hepatocellular carcinoma: An immunohistochemical study
Expression of vascular endothelial growth factor in normal liver and hepatocellular carcinoma: An immunohistochemical study
复制标题
DOI：
10.1016/s0046-8177(97)90179-9
复制
发表时间：
1997-06-01
期刊：
影响因子：
3.3
通讯作者：
Su, IJ
中科院分区：
文献类型：
--
作者：
Chow, NH;Hsu, PI;Su, IJ
血管生成在实体瘤的发生发展过程中起着至关重要的作用。为探讨血管内皮生长因子(VEGF)在肝细胞癌发生中的作用，我们检测了6例正常人肝组织和36例肝细胞癌(HCC)组织中血管内皮生长因子(VEGF)的表达。在正常肝和非肿瘤肝组织中，血管内皮生长因子免疫反应阳性表达于汇管管细胞外基质中，而在肝细胞和胆管上皮细胞中未见表达。在肝细胞癌中，13例(36.1%)肿瘤细胞表达不同数量的血管内皮生长因子。Logistic回归模型显示，血管内皮生长因子表达与较高的增殖指数(P=.01)和超声门静脉血栓形成(P=.05)显著相关。然而，血管内皮生长因子的表达与生化肝功能、甲胎蛋白水平、组织学分级、性别或肝硬变的临床分期无关(分别为P&gT；0.1)。对数等级检验显示，与肿瘤体积和门静脉血栓形成(P<；.01)相比，血管内皮生长因子不能提供更多的预后信息(P>；.5)。此外，在肝细胞癌中，血管内皮生长因子始终存在于纤维血管间质或细胞周围基质中，但与肿瘤细胞中血管内皮生长因子的表达无明显相关性(P>；5)。我们的数据提示，在体内发生肝癌的过程中，血管内皮生长因子的表达可能是向高增殖方向发展的特征。正常肝和肝细胞癌的细胞外基质中是否存在血管内皮生长因子的相关性尚不清楚。版权所有(C)1997，由W.B.Saunders公司提供。
Angiogenesis is of vital importance during the development and progression of solid tumors. To examine the role of vascular endothelial growth factor (VEGF) in hepatocarcinogenesis, we evaluated the expression of peptide in normal human liver (n = 6) and in 36 cases of hepatocellular carcinoma (HCC). Immunoreactivity for VEGF was present in the extracellular matrix of the portal tracts in the normal and nontumor part of liver, but not in hepatocytes and bile duct epithelium. For HCC, variable amounts of VEGF were expressed in 13 cases (36.1%) of tumor cells. Using a logistic regression model, expression of VEGF was significantly associated with a higher proliferative index (P = .01) and sonographic portal vein thrombosis (P = .05). However, VEGF expression did not correlate with a biochemical liver profile, alpha-fetoprotein levels, histological grading, gender, or clinical stage of cirrhosis (P > 0.1, respectively). Log-rank test showed that evaluation of VEGF did not provide more prognostic information (P > .5) than that from tumor volume and portal vein thrombosis (P < .01, respectively). In addition, VEGF was always present in the fibrovascular stroma or pericellular matrix of HCC, although no strong relationship was observed with the expression of VEGF in tumor cells (P > .5). Our data suggested that expression of VEGF may characterize a progression toward higher proliferation in hepatocarcinogenesis in vivo. The relevance of VEGF existing in the extracellular matrix of the normal liver and HCC remains to be clarified. Copyright (C) 1997 by W.B. Saunders Company.