Monoclonal antibody (5F3) defining renal cell carcinoma-associated antigen disialosyl globopentaosylceramide (V3NeuAcIV6NeuAcGb5), and distribution pattern of the antigen in tumor and normal tissues.
Monoclonal antibody (5F3) defining renal cell carcinoma-associated antigen disialosyl globopentaosylceramide (V3NeuAcIV6NeuAcGb5), and distribution pattern of the antigen in tumor and normal tissues.
复制标题
单克隆抗体 (5F3) 定义肾细胞癌相关抗原二唾液酸基球五糖神经酰胺 (V3NeuAcIV6NeuAcGb5) 以及该抗原在肿瘤和正常组织中的分布模式。
DOI：
10.1023/a:1016281002344
复制
发表时间：
2001
影响因子：
3
通讯作者：
Hakomori,SI
中科院分区：
文献类型：
--
作者：
Ito,A;Saito,S;Masuko,T;Oh-Eda,M;Matsuura,T;Satoh,M;Nejad,FM;Enomoto,T;Orikasa,S;Hakomori,SI
肾细胞癌(RCC)的特点是高表达三种类型的二唾液酸神经节苷脂：两种基于乳糖系列的1型结构(DIsialosyl LC4，GalNAc Disialosyl LC4)，另一种基于Globo系列结构(Dsialosyl lobentaosylceramide；Dsialosyl GB5)。本研究建立了针对二唾液酸基GB5的单抗5F3。用肾癌细胞株ACHN免疫后，建立了5F3。从ACHN细胞中分离出与5F3特异反应的主要二唾液酸神经节苷脂抗原，经酶和酸解后的核心结构鉴定为球状戊糖神经酰胺(GB5)，并经二维1H-核磁共振波谱分析，确定为二唾液酸基GB5(V3NeuAcIV6NeuAcGb5)。5F3不与单唾液酸基GB5(V3NeuAcGb5)、GB5或任何内酯系列结构反应。41例原发肾细胞癌组织中19例5F3强染。它与肾脏近端小管(而不是远端小管)、大脑和小脑的小胶质细胞、胃肠的杯状细胞、各器官的平滑肌呈阳性反应。除肾上皮和前列腺间质外，不与其他器官的实质细胞反应。用单抗5F3对肾癌组织进行免疫染色，结合其他针对球系和乳链结构的抗体进行染色，对判断肾癌的转移潜能有一定的预后意义。
Renal cell carcinoma (RCC) has been characterized by high expression of three types of disialogangliosides: two based on lacto-series type 1 structure (disialosyl Lc4, GalNAc disialosyl Lc4), the other based on globo-series structure (disialosyl globopentaosylceramide; disialosyl Gb5). The present study established a mAb, 5F3, directed to disialosyl Gb5. 5F3 was established after immunization with RCC cell line ACHN. The major disialoganglioside antigen isolated from ACHN cells, showing specific reactivity with 5F3, was characterized unequivocally as disialosyl Gb5 (V3NeuAcIV6NeuAcGb5) by identification of the core structure as globopentaosylceramide (Gb5) after enzymatic and acid hydrolysis, and by 2-dimensional1H-NMR spectroscopy. 5F3 does not react with monosialosyl Gb5 (V3NeuAcGb5), Gb5, or any lacto-series structures. 5F3 strongly stained 19 of 41 cases of primary RCC tissue. It reacted with proximal tubules (but not distal tubules) of kidney, microglial cells of cerebrum and cerebellum, goblet cells of stomach and intestine, smooth muscle of various organs. It did not react with parenchymatous cells of various organs, except for kidney epithelia and prostate stroma. Immunostaining of RCC tissue by mAb 5F3, in combination with staining by other antibodies directed to globo-series and lacto-series structures, has prognostic significance in defining metastatic potential of RCC.