Association of GST genotypes with age of onset and lymph node metastasis in oral squamous cell carcinoma.
Association of GST genotypes with age of onset and lymph node metastasis in oral squamous cell carcinoma.
复制标题
GST 基因型与口腔鳞状细胞癌发病年龄和淋巴结转移的关系。
DOI：
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发表时间：
2005
影响因子：
3.3
通讯作者：
Kuo
中科院分区：
文献类型：
--
作者：
Chung;Che‐Shoa Chang;Man;C. Dang;Y. Shih;Kuo
背景
参与致癌物代谢的 I 相和 II 相酶充分证明了口腔致癌过程中环境与基因的相互作用。本研究调查了 II 期酶基因的谷胱甘肽 S-转移酶 (GST)T1 和 GSTM1 基因型与槟榔相关口腔鳞状细胞癌 (OSCC) 的发病年龄和颈部淋巴结转移 (LNM) 的风险之间的关系。
方法
总共招募了 114 名 OSCC 男性患者和 100 名男性对照。所有受试者都是槟榔使用者和吸烟者。 DNA 是从外周血样本中获得的。 GSTT1（非无效/无效）和 GSTM1（非无效/无效）的基因分型是使用仅扩增非无效等位基因的特异性引物通过聚合酶链反应 (PCR) 分析确定的。
结果
根据病例对照，未发现 GST 基因型与 OSCC 风险之间存在关联。 GSTT1 无效基因型的患者发病年龄较大 (P = 0.03)。 GSTM1 无效基因型患者的颈部 LNM 发生率高于 GSTM1 非无效基因型患者（P = 0.01）。与携带相反基因型的患者相比，GSTM1/GSTT1 无效基因型的患者似乎发病较晚，且颈部 LNM 的发生率较高。
结论
GST 基因型可能是 OSCC 发病年龄和转移风险的重要标志。数据还表明，各种 GST 亚型可能不同程度地参与 OSCC 的发生或进展。
BACKGROUND
Environment-gene interaction in oral carcinogenesis is well demonstrated by phase I and II enzymes that are involved in the metabolism of carcinogens. This study investigated the association of glutathione S-transferase (GST)T1 and GSTM1 genotypes of phase II enzyme genes with risk for, age of onset, and neck lymph node metastasis (LNM) in areca-associated oral squamous cell carcinoma (OSCC).
METHODS
A total of 114 OSCC male patients and 100 male controls were recruited. All subjects were areca users and tobacco smokers. DNA was obtained from peripheral blood samples. Genotyping of GSTT1 (non-null/null) and GSTM1 (non-null/null) was determined by polymerase chain reaction (PCR) analysis using specific primers that only amplify non-null alleles.
RESULTS
No association was found between GST genotype and the risk of OSCC based on case-controls. Patients with the GSTT1 null genotype were older at onset (P = 0.03). Those with the GSTM1 null genotype had a higher incidence of neck LNM than those with the GSTM1 non-null genotype (P = 0.01). Patients with the GSTM1/GSTT1 null genotype appeared to have later onset and a higher incidence of neck LNM than those carrying the opposite genotype.
CONCLUSION
The GST genotypes may be important markers for the age of onset and risk of metastasis in OSCC. The data also suggest that the various GST isoforms may be differentially involved in development or progression of OSCC.
影响因子：
5.3
作者：
Yang, P;Yokomizo, A;Liu, WG
通讯作者：
Liu, WG