Effect of Hypogymnia physodes Extracts and their Depsidones on Micronucleus Distribution in Human Lymphocytes
Effect of Hypogymnia physodes Extracts and their Depsidones on Micronucleus Distribution in Human Lymphocytes
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DOI：
10.1177/1934578x1300800125
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发表时间：
2013-01
影响因子：
1.8
通讯作者：
I. Stojanović;M. Stankovic;O. Jovanovic;G. Petrovic;A. Šmelcerović;G. Stojanović
中科院分区：
文献类型：
--
作者：
I. Stojanović;M. Stankovic;O. Jovanovic;G. Petrovic;A. Šmelcerović;G. Stojanović
用柱层析法从Hypogymnia physodes乙醚提取物中分离得到3个地衣缩酚酮化合物，分别为Physodalic acid（1）、Physodic acid（2）和3-hydroxy Physodic acid（3）。通过UV、1H和13 C NMR和MS数据与文献及CHEM draw ultra 11软件计算数据的比较，确定了它们的结构。采用反相高效液相色谱-光电二极管阵列检测器测定了甲醇（ME）、丙酮（AE）和乙醚（EE）提取物中1、2和3的含量。采用细胞松弛素B阻断微核（CBMN）试验，以1 μg/mL和2 μg/mL最终培养液的剂量，评价了提取物、分离的缩酚酮1-3和EE组分F23（由90%的2和3组成，比例为5.5：1）对人外周血淋巴细胞染色体畸变的体外影响。计数双核细胞微核率，计算核增殖指数。发现1.0 μg/mL的1、2、3、F23和EE对淋巴细胞产生有益作用，与阳性对照Amifostin WR-2721相比，MN频率显著降低。在浓度为1 μg/mL的供试缩酚酮中，3种缩酚酮表现出最显著的降低MN频率的效果，降低幅度为30.3%，其次是2种缩酚酮（28.2%）和1种缩酚酮（22.0%）。提取物的有效性低于分离的缩酚酮。
Three lichen depsidones, physodalic acid (1), physodic acid (2), and 3-hydroxy physodic acid (3), were isolated from Hypogymnia physodes diethyl ether extract using column chromatography, and their structures determined by comparing their UV,1H and 13C NMR spectroscopic and MS data with those given in the literature, as well as with data computed by CHEM draw ultra 11 software. The contents of 1, 2 and 3 were determined in the methanol (ME), acetone (AE), and diethyl ether (EE) extracts using reversed-phase high performance liquid chromatography with photodiode array detection. The extracts, isolated depsidones 1-3 and EE fraction F23 (consisting of 90% 2 and 3, in the ratio 5.5: 1) were evaluated for their in vitro effects on chromosome aberrations in peripheral human lymphocytes using the cytochalasin-B blocked micronucleus (CBMN) assay in doses of 1 μg/mL and 2 μg/mL of final culture solution. The frequency of MN was scored in binucleated cells, and nuclear proliferation index was calculated. It was found that 1, 2, 3, F23, and EE at 1.0 μg/mL exerted a beneficial effect on lymphocyte cells giving a significant decrease of the frequency of MN in comparison with the positive control Amifostin WR-2721. Among the tested depsidones at a concentration of 1 μg/mL, 3 exhibited the most prominent effect decreasing the frequency of MN by 30.3%, followed by 2 (28.2%) and 1 (22.0%). The extracts were less effective than the isolated depsidones.