High‐affinity immunoglobulin E receptor (FcεRI)‐bearing eosinophils, mast cells, macrophages and Langerhans’ cells in allergen‐induced late‐phase cutaneous reactions in atopic subjects
High‐affinity immunoglobulin E receptor (FcεRI)‐bearing eosinophils, mast cells, macrophages and Langerhans’ cells in allergen‐induced late‐phase cutaneous reactions in atopic subjects
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特应性受试者过敏原诱导的晚期皮肤反应中携带高亲和力免疫球蛋白 E 受体 (FcεRI) 的嗜酸性粒细胞、肥大细胞、巨噬细胞和朗格汉斯细胞
DOI：
10.1046/j.1365-2567.1998.00418.x
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发表时间：
1998
期刊：
影响因子：
6.4
通讯作者：
Kay
中科院分区：
文献类型：
--
作者：
Ying;Barata;Meng;Grant;Barkans;Durham;Kay
我们使用原位杂交（ISH）和免疫组织化学（IHC）研究了过敏性受试者过敏原诱导的晚期皮肤反应中FcεRI mRNA（α、β和γ链）、α链蛋白产物的表达动力学以及mRNA或蛋白阳性细胞的表型。与稀释液对照相比，过敏原激发后所有时间点（6、24和48 h）FcεRI α-、β-和γ-链的mRNA+细胞总数均显著增加（P<0·01）。 免疫组化法检测过敏原刺激后巨噬细胞、嗜酸性粒细胞、肥大细胞和CD 1a+细胞α、β和γ链mRNA阳性细胞数均显著增加（P<0.05）。FcεRI亚基（α-、β-或γ-链）mRNA+共定位分布为CD 68+巨噬细胞（42-47%）、EG 2+嗜酸性粒细胞（33-39%）、类胰蛋白酶+肥大细胞（5-11%）和CD 1a+朗格汉斯细胞（2-4%）。免疫组化结果显示，在过敏原刺激后24和48 h，FcεRI蛋白阳性细胞总数显著增加（P<0.01）。双重IHC显示，在24小时过敏原激发部位，FcεRI+细胞的分布为类胰蛋白酶+肥大细胞（33%）、CD 68+巨噬细胞（36%）、EG 2+嗜酸性粒细胞（20%）、CD 1a+朗格汉斯细胞（4%）和未识别细胞（7%）。这些观察结果表明，人体皮肤晚期反应与嗜酸性粒细胞、巨噬细胞、肥大细胞和朗格汉斯细胞上FcεRI的上调相关。
We have used in situ hybridization (ISH) and immunohistochemistry (IHC) to investigate the kinetics of the expression for FcεRI mRNA (α‐, β‐ and γ‐chains), the α‐chain protein product, as well as the phenotype of the mRNA‐ or protein‐positive cells in allergen‐induced late‐phase skin reactions in atopic subjects. Compared with diluent controls, there were significant increases in the total numbers of mRNA+ cells for the α‐, β‐and γ‐chains for FcεRI at all time‐points (6, 24 and 48 hr) after allergen challenge (P<0·01). By double IHC/ISH significant increases in α‐, β‐ and γ‐chain mRNA+ macrophages, eosinophils, mast cells and CD1a+ cells were also observed after allergen challenge (P<0·05). The distribution of FcεRI subunit (α‐, β‐, or γ‐chain) mRNA+ co‐localization was CD68+ macrophages (42–47%), EG2+ eosinophils (33–39%), tryptase+ mast cells (5–11%) and CD1a+ Langerhans’ cells (2–4%). Using single IHC, significant increases in the total number of FcεRI protein+ cells (P<0·01) were observed 24 and 48 hr after allergen challenge. Double IHC showed that the distribution of FcεRI+ cells was tryptase+ mast cells (33%), CD68+ macrophages (36%), EG2+ eosinophils (20%), CD1a+ Langerhans’ cells (4%) and unidentified cells (7%), at the 24‐hr allergen‐challenged sites. These observations suggest that the cutaneous late‐phase reaction in man is associated with up‐regulation of FcεRI on eosinophils, macrophages, mast cells and Langerhans’ cells.
影响因子：
4.4
作者：
P. Weller;T. H. Rand;T. Barrett;A. Elovic;David T. W. Wong;R. Finberg
通讯作者：
P. Weller;T. H. Rand;T. Barrett;A. Elovic;David T. W. Wong;R. Finberg
影响因子：
14.2
作者：
BROIDE, DH;GLEICH, GJ;WASSERMAN, SI
通讯作者：
WASSERMAN, SI