ENHANCEMENT OF HUMAN GRANULOPOIESIS INVITRO BY BIOSYNTHETIC INSULIN-LIKE GROWTH FACTOR-I SOMATOMEDIN-C AND HUMAN GROWTH-HORMONE
ENHANCEMENT OF HUMAN GRANULOPOIESIS INVITRO BY BIOSYNTHETIC INSULIN-LIKE GROWTH FACTOR-I SOMATOMEDIN-C AND HUMAN GROWTH-HORMONE
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DOI：
10.1172/jci113385
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发表时间：
1988-03-01
影响因子：
15.9
通讯作者：
HOCHBERG, Z
中科院分区：
文献类型：
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作者：
MERCHAV, S;TATARSKY, I;HOCHBERG, Z
研究了生物合成的重组胰岛素样生长因子I/生长原子素C(IGF-I/Sm-C)和人生长激素(HGH)对人骨髓祖细胞体外生长和成熟的影响。60 ng/mlIGF-I/Sm-C和250 ng/mlhGH可最大限度地促进髓系集落形成，使集落数增加41.+-。7和38。+-。4%(P<；0.001)。两种多肽均可诱导单核细胞集落形成频率增加1.5-2.5倍，但不改变单核/巨噬细胞或混合粒/巨噬细胞集落的数量。在人骨髓细胞悬浮培养中，IGF-I/Sm-C和hGH也能促进髓系向成熟粒细胞的成熟。在人重组粒细胞/巨噬细胞集落刺激因子刺激的无血清培养中，这两种多肽对人骨髓粒细胞生成的影响也同样明显。HGH对体外培养的人骨髓粒细胞的促进作用需要骨髓贴壁细胞的存在，但这种作用可被针对IGF-I/Sm-C受体的特异性单抗所阻断。因此，hGH对骨髓髓系祖细胞的作用似乎是通过旁分泌IGF-I/Sm-C介导的。
The effect of biosynthetic recombinant insulin-like growth factor I/somatomedin C (IGF-I/Sm-C) and human growth hormone (hGH) on the in vitro growth and maturation of human marrow myeloid progenitors was investigated. Myeloid colony formation was maximally enhanced by 60 ng/ml IGF-I/Sm-C and by 250 ng/ml hGH, resulting in an increase in colony numbers of 41 .+-. 7 and 38 .+-. 4%, respectively (P < 0.001). Both peptides induced a 1.5-2.5-fold increase in the frequency of colonies composed of granulocytes alone, but did not alter the numbers of monocyte/macrophage or mixed granulocyte/macrophage colonies. IGF-I/Sm-C and hGH were also found to enhance myeloid maturation towards mature granulocytes in suspension cultures of human marrow cells. The effect of both peptides on human marrow granulopoiesis was similarly demonstrable in serum-free cultures stimulated with human recombinant granulocyte/macrophage colony-stimulating factor. Enhancement of human marrow granulopoiesis in vitro by hGH required the presence of marrow adherent cells and was abrogated by specific monoclonal antibodies directed against IGF-I/Sm-C receptors. The effect of hGH on marrow myeloid progenitors thus appears to be mediated by paracrine IGF-I/Sm-C.