CLARITY for mapping the nervous system
CLARITY for mapping the nervous system
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DOI：
10.1038/nmeth.2481
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发表时间：
2013-06-01
期刊：
影响因子：
48
通讯作者：
Deisseroth, Karl
中科院分区：
文献类型：
--
作者：
Chung, Kwanghun;Deisseroth, Karl
与脑映射工作的潜在相关性，现在可以从生物组织内构建基于水凝胶的结构，以允许随后在不机械分解组织的情况下去除脂质。这个过程产生了一种物理稳定的组织-水凝胶混合物，它保留了精细结构、蛋白质和核酸，并且对可见光谱光子和外源性大分子都是可渗透的。H在这里，我们强调了这种方法的相关挑战和机遇，特别是在与脑成像研究的互补方法的整合方面。
With potential relevance for brain-mapping work, hydrogel-based structures can now be built from within biological tissue to allow subsequent removal of lipids without mechanical disassembly of the tissue. T This process creates a tissue-hydrogel hybrid that is physically stable, that preserves fine structure, proteins and nucleic acids, and that is permeable to both visible-spectrum photons and exogenous macromolecules. H Here we highlight relevant challenges and opportunities of this approach, especially with regard to integration with complementary methodologies for brain-mapping studies.