Antiasthmatic activity and selective inhibition of type 2 helper T cell response by aqueous extract of semen armeniacae amarum
Antiasthmatic activity and selective inhibition of type 2 helper T cell response by aqueous extract of semen armeniacae amarum
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DOI：
10.1080/08923970600815253
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发表时间：
2006-04-01
影响因子：
3.3
通讯作者：
Nam, Sang-Yun
中科院分区：
文献类型：
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作者：
Do, Jeong-Su;Hwang, Jin-Ki;Nam, Sang-Yun
在韩国传统医学中，杏仁（SAA）长期被用于控制哮喘。然而，其抗哮喘作用仍然知之甚少。本研究通过反复致敏和鼻内攻击OVA诱导的小鼠过敏性哮喘模型，探讨SAA的作用机制。通过β-乙酰甲胆碱诱导的气流阻塞和包括嗜酸性粒细胞在内的白细胞的气道募集测量的气道高反应性（AHR）通过口服SAA水提取物治疗显著降低。支气管肺泡灌洗液（BALF）中的白细胞介素（IL）-4水平也明显低于对照组，而干扰素γ（IFN-γ）水平则无明显变化。总的来说，这些数据表明SAA抑制2型辅助性T细胞（Th 2），但不抑制1型辅助性T细胞（Th 1）应答。支气管周围淋巴结细胞的离体细胞因子产生的数据进一步支持了这一假设。因此，SAA口服给药可减轻哮喘表现，包括AHR和气道炎症，这可能是由于选择性抑制Th 2对变应原的反应。我们的数据有力地表明，SAA可以有效地应用于控制其他Th 2相关疾病以及过敏性哮喘。
Semen armeniacae amarum (SAA) has long been used to control asthma in Korean traditional medicine. However, its antiasthmatic action still remains poorly understood. In the current study, effective mechanism of SAA was investigated in a mouse model of allergic asthma induced by repeated sensitization and intranasal challenge with OVA. Airway hyperreactivity (AHR) measured by beta-methacoline-induced airflow obstruction and airway recruitment of leukocytes including eosinophils were significantly reduced by oral treatment of SAA water extract. Level of interleukin (IL)-4, but not Interferon gamma (IFN-gamma), in the bronchoalveolar lavage fluid (BALF) also appeared considerably lower in SAA-treated mice than in controls. Collectively, these data show that SAA suppresses type 2 helper T cell (Th2), but not type 1 helper T cell (Th1), response. This hypothesis was supported further by the data of ex vivo cytokine production of peribronchial lymph node cells. Thus, oral administration of SAA attenuates asthmatic manifestations including AHR and airway inflammation, which possibly result from selective inhibition of Th2 response to allergen. Our data strongly suggest that SAA may be effectively applied to control other Th2-related diseases as well as allergic asthma.