Genome-Wide Detection of Meiotic DNA Double-Strand Break Hotspots Using Single-Stranded DNA
Genome-Wide Detection of Meiotic DNA Double-Strand Break Hotspots Using Single-Stranded DNA
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DOI：
10.1007/978-1-61779-129-1_4
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发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Hochwagen, Andreas
中科院分区：
文献类型：
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作者：
Blitzblau, Hannah G.;Hochwagen, Andreas
The controlled fragmentation of chromosomes by DNA double-strand breaks (DSBs) initiates meiotic recombination, which is essential for meiotic chromosome segregation in most eukaryotes. This chapter describes a straightforward microarray-based approach to measure the genome-wide distribution of meiotic DSBs by detecting the single-stranded DNA (ssDNA) that transiently accumulates at DSB sites during recombination. The protocol outlined here has been optimized to detect meiotic DSBs in Saccharomyces cerevisige. However, because ssDNA is a universal intermediate of homologous recombination, this method can ostensibly be adapted to discover and analyze programmed or damage-induced DSB hotspots in other organisms whose genome sequence is available.
