Expression of ER stress and autophagy-related molecules in human non-small cell lung cancer and premalignant lesions
Expression of ER stress and autophagy-related molecules in human non-small cell lung cancer and premalignant lesions
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DOI：
10.1002/ijc.26463
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发表时间：
2012-08-15
影响因子：
6.4
通讯作者：
Chung, Myoung Ja
中科院分区：
文献类型：
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作者：
Kim, Kyoung Min;Yu, Tack Keun;Chung, Myoung Ja
损害内质网(ER)功能的应激导致内质网中未折叠或错误折叠的蛋白质的积累(ER应激)。自噬是一种溶酶体途径，参与细胞大分子和细胞器的周转，新出现的数据表明内质网应激也是自噬的有力诱导者。内质网压力和自噬与人类癌症有关。我们检测了内质网应激相关蛋白[GRP78和C/EBP同源蛋白(CHOP)]和自噬蛋白(Beclin-1和LC3)在非小细胞肺癌(NSCLC)、细支气管肺泡癌(BAC)和非典型腺瘤性增生症(AAHS)中的表达，以了解它们在NSCLC发病机制中的作用。应用免疫组织化学方法检测133例非小细胞肺癌(69例鳞癌、56例腺癌和8例其他非小细胞肺癌)、21例BAC和9例AAH组织中GRP78、CHOP、Beclin-1和LC3的表达。GRP78和Beclin-1的表达与肿瘤的低分期(p<；0.001和p=0.019)和较长的生存期(p=0.007和p=p
Stress that impairs endoplasmic reticulum (ER) function leads to an accumulation of unfolded or misfolded proteins in the ER (ER stress). Autophagy is a lysosomal pathway involved in the turnover of cellular macromolecules and organelles, which emerging data indicate that ER stress is also a potent inducer of autophagy. ER stress and autophagy are involved in human cancer. We examined the expression of ER stress-related proteins [GRP78 and C/EBP homologous protein (CHOP)] and autophagic proteins (Beclin-1 and LC3) in non-small cell lung carcinomas (NSCLCs), bronchioloalveolar carcinomas (BACs) and atypical adenomatous hyperplasias (AAHs) to understand their role in the NSCLC pathogenesis. The expression of GRP78 and CHOP, Beclin-1 and LC3 were analyzed using immunohistochemistry on tissue sections from 133 NSCLC (69 squamous cell carcinomas, 56 adenocarcinomas (AC) and eight other NSCLCs), 21 BAC and 9 AAH. Expression of GRP78 and Beclin-1 was correlated with low tumor stage (p < 0.001 and p = 0.019, respectively) and longer survival (p = 0.007 and p