Molecular cloning of a human cDNA for the 41-kDa phosphoribosylpyrophosphate synthetase-associated protein
Molecular cloning of a human cDNA for the 41-kDa phosphoribosylpyrophosphate synthetase-associated protein
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DOI：
10.1016/s0167-4781(97)00217-0
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发表时间：
1998-03-13
期刊：
影响因子：
--
通讯作者：
Itakura, M
中科院分区：
文献类型：
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作者：
Katashima, R;Iwahana, H;Itakura, M
从两个表达序列标签（EST）克隆中克隆了编码41-kDa磷酸核糖焦磷酸（PRPP）合成酶（PRS）相关蛋白（PAP 41）的人cDNA，所述表达序列标签（EST）克隆与人PAP 39 cDNA具有61.5%和70.0%的核苷酸相似性。预测的开放阅读框架为1107个碱基对（bp），与大鼠PAP 41的核苷酸同一性为91.8%，编码369个氨基酸的蛋白质，计算的分子量（MW）为40，925。推导的氨基酸序列与大鼠PAP 41的同源性为98.9%，与人PAP 39、PRS I和PRS II的同源性分别为72.2%、50.6%和50.0%，但缺少PRPP结合位点。Southern印迹分析表明，PAP 41基因在人类基因组中以单拷贝存在。北方印迹分析显示，在5个人细胞系中存在约2.1kb的单个PAP 41 mRNA。(C)1998年Elsevier Science B.V.
A human cDNA encoding 41-kDa phosphoribosylpyrophosphate (PRPP) synthetase (PRS)-associated protein (PAP41) was cloned from two expressed sequence tag (EST) clones having the nucleotide similarity of 61.5 and 70.0% to human PAP39 cDNA. The predicted open reading frame of 1107 base pairs (bp) has the nucleotide identity of 91.8% to rat PAP41 and encodes a protein of 369 amino acids with a calculated molecular weight (MW) of 40,925. The deduced amino acid sequence exhibits the 98.9% identity to rat PAP41 and 72.2, 50.6, and 50.0% identity with human PAP39, PRS I, and PRS II, respectively, but lacks the PRPP binding site. Southern blot analysis suggested that the PAP41 gene exists as a single copy in the human genome. The single PAP41 mRNA of about 2.1 kb was shown to be present in five human cell lines by Northern blot analysis. (C) 1998 Elsevier Science B.V.