The relationship between IGF1 and the expression spectrum of miRNA in the placenta of preeclampsia patients
The relationship between IGF1 and the expression spectrum of miRNA in the placenta of preeclampsia patients
复制标题
子痫前期患者胎盘中IGF1与miRNA表达谱的关系
DOI：
10.5603/gp.2019.0096
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发表时间：
2019-01-01
影响因子：
1.3
通讯作者：
Fu, Fen
中科院分区：
文献类型：
--
作者：
He, Xiaoju;Zhao, Lu;Fu, Fen
目的：先兆子痫（PE）影响全世界许多妇女，仍然是新生儿和孕产妇发病和死亡的主要原因。胎盘microRNAs （miRNAs）的异常表达可能与PE有关。材料与方法：本研究旨在探讨子痫前期患者胎盘中IGF1与miRNA表达谱的关系。比较子痫前期（n = 6）和正常孕妇（n = 5）胎盘组织中miRNA的表达情况，通过计算机模拟和功能分析研究miRNA靶点。通过细胞凋亡实验和侵袭实验验证了miRNA在滋养细胞中的作用。结果：子痫前期患者胎盘mirna含量明显高于正常妊娠患者。荧光素酶测定证实了miRNA的直接调控。结论：子痫前期患者胎盘中IGF1和miRNA的表达明显升高。
Objectives: Pre-eclampsia (PE) affects many women worldwide and remains the leading cause of morbidity and mortality in neonatal and maternal settings. Abnormal expression of placental microRNAs (miRNAs) may be associated with PE.Material and methods:This study was conducted to the relationship between IGF1 and the expression spectrum of miRNA in the placenta of preeclampsia patient.The expression of miRNA in placental tissue was compared between pre-eclampsia (n = 6) and normal pregnant women (n = 5) miRNA targets were studied by computer simulation and functional assays.The role of miRNA was verified in trophoblast cell lines by apoptosis assay and invasion assay.Results: There was a significant increase in miRNAs in the placenta of women with pre-eclampsia compared with patients with normal pregnancy. Luciferase assay confirmed direct regulation of miRNA.Conclusions:The expression of IGF1 and miRNA was significantly increased in the placenta of patients with pre-eclampsia.