MOBILE ELEMENTS BOUNDED BY C4A4 TELOMERIC REPEATS IN OXYTRICHA FALLAX
MOBILE ELEMENTS BOUNDED BY C4A4 TELOMERIC REPEATS IN OXYTRICHA FALLAX
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DOI：
10.1016/0092-8674(85)90249-1
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发表时间：
1985-12-01
期刊：
影响因子：
64.5
通讯作者：
WILLIAMS, K
中科院分区：
文献类型：
--
作者：
HERRICK, G;CARTINHOUR, S;WILLIAMS, K
描述了一个新的微核元件家族，称为端粒承载元件（TBE）。所有1900个家庭成员在大核发育过程中被淘汰。我们的结论是，他们是转座子，首先是因为成员是适度保守的序列，并可能分散在基因组中。第二，在两种情况下，元件的末端和侧翼与相应的空位点的序列比较表明，元件在插入时引起3bp靶重复（AAT）; 3bp是5 bp靶序列AATGA的一部分。最后，两个元件都携带77或78 bp的反向末端重复序列。每个反向末端重复的末端是17 bp的端粒样序列5“C1 A4 C4 A4 C4。至少有一半的元件具有这17 bp或极其相似的序列。转座进入新的微核位点的一个可能途径是从发育中的大核开始，切除以产生一个自由的线性形式，端粒被添加到其中，然后低频率地移动到微核，并插入到生殖系微核DNA中。
A novel family of micronuclear elements termed telomere-bearing elements (TBEs) is described. All 1900 family members are eliminated during macronuclear development. We conclude that they are transposons, first because the members are moderately conserved in sequence and probably dispersed in the genome. Second, in two cases, sequence comparision of the termini and flanks of the element with the corresponding empty site indicate that elements cause 3 bp target duplications (AAT) upon insertion; the 3 bp are part of the 5 bp target sequence, AATGA. Lastly, both elements carry 77 or 78 bp inverted terminal repeats. The tip of each inverted terminal repeat is the 17 bp telomere-like sequence 5'' C1A4C4A4C4. At least half of the elements have these 17 bp or an extremely similar sequence. One possible pathway for transpositon into new micronuclear sites starts in the developing macronucleus with excision to create a free linear form to which telomeres are added, followed by a low frequency of movement to the micronucleus, and insertion into the germ-line micronuclear DNA.