Dietary grape seed proanthocyanidins improved growth, immunity, antioxidant, digestive enzymes activities, and intestinal microbiota of juvenile hybrid sturgeon ( Acipenser baeri Brandt ♀ × A. schrenckii Brandt ♂)
Dietary grape seed proanthocyanidins improved growth, immunity, antioxidant, digestive enzymes activities, and intestinal microbiota of juvenile hybrid sturgeon ( Acipenser baeri Brandt ♀ × A. schrenckii Brandt ♂)
复制标题
DOI：
10.1111/anu.13334
复制
发表时间：
2021-09
影响因子：
3.5
通讯作者：
Guanling Xu;W. Xing;Tieliang Li;Huanhuan Yu;Shibo Wei;Na Jiang;Zhihong Ma;Lin Luo
中科院分区：
文献类型：
--
作者：
Guanling Xu;W. Xing;Tieliang Li;Huanhuan Yu;Shibo Wei;Na Jiang;Zhihong Ma;Lin Luo
通过为期 8 周的生长试验，评估葡萄籽原花青素 (GSP) 对杂交鲟幼鱼 (Acipenser baeriBrandt ♀ × A. schrenckiiBrandt ♂) 生长、消化酶、身体成分、肠道微生物群、血清免疫、抗氧化和生化参数的影响。将鱼随机分为对照组、GSPs-50 组和 GSPs-100 组，分别饲喂基础饲料和添加 50 毫克/千克和 100 毫克/千克 GSP 的基础饲料。 GSP 改善了鲟鱼的生长性能和消化酶 (p< 0.05)。当鲟鱼饲喂 GSP 饲料时，血清中的溶菌酶明显增加，而硫代巴比妥酸反应物质 (TBARS) 则表现出相反的趋势 (p< 0.05)。 GSPs-50 血清中的髓过氧化物酶和谷胱甘肽过氧化物酶高于对照和 GSPs-100 (p< .05)。血清参数显示 GSPs-50 的丙氨酸氨基转移酶、碱性磷酸酶和 γ-谷氨酰转肽酶低于对照 (p< 0.05)。与对照处理相比，GSPs-100 降低了丙氨酸转氨酶和 γ-谷氨酰转肽酶，并增加了天冬氨酸转氨酶和乳酸脱氢酶 (p<.05)。高通量测序显示，添加 50 mg/kg GSP 不仅改变了微生物群多样性，而且还降低了鲟鱼肠道中气单胞菌素的相对丰度。总之，GSPs是一种很有前景的饲料添加剂，可促进杂交鲟的生长、免疫、抗氧化和消化酶活性，改善血清生化参数和肠道微生物群。
An 8‐week growth trial was performed to evaluate the effects of grape seed proanthocyanidins (GSPs) on growth, digestive enzymes, body composition, intestinal microbiota, serum immunological, anti‐oxidative and biochemical parameters of juvenile hybrid sturgeon (Acipenser baeriBrandt ♀ ×A. schrenckiiBrandt ♂). Fish were randomly divided to control, GSPs‐50 and GSPs‐100 groups, which were fed with a basal diet and basal diet supplemented with 50 and 100 mg/kg GSPs, respectively. Growth performance and digestive enzymes were improved by GSPs in sturgeon (p< .05). Lysozyme was clearly increased in serum when sturgeon fed with GSPs diets, while the thiobarbituric acid reactive substances (TBARS) displayed opposite trend (p< .05). Myeloperoxidase and glutathione peroxidase in serum of GSPs‐50 were higher than control and GSPs‐100 (p< .05). Serum parameters showed alanine aminotransferase, alkaline phosphatase and γ‐glutamyl transpeptidase of GSPs‐50 were lower than control (p< .05). Compared with the control treatment, GSPs‐100 reduced alanine aminotransferase and γ‐glutamyl transpeptidase and increased aspartate aminotransferase and lactate dehydrogenase (p< .05). High‐throughput sequencing revealed that adding 50 mg/kg GSPs not only modified the microbiota diversity, but also reduced the relative abundance ofAeromonasin sturgeon intestine. In conclusion, GSPs are a promising feed additive to promote growth, immunity, antioxidant and digestive enzyme activities and improve serum biochemical parameters and intestinal microbiota of hybrid sturgeon.