Modulation of monocyte chemotactic protein-1 expression during lipopolysaccharide-induced preterm delivery in the pregnant mouse
Modulation of monocyte chemotactic protein-1 expression during lipopolysaccharide-induced preterm delivery in the pregnant mouse
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DOI：
10.1177/1933719107307792
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发表时间：
2007-09-01
影响因子：
2.9
通讯作者：
Phillippe, Mark
中科院分区：
文献类型：
--
作者：
Diamond, Allaire K.;Sweet, Leigh M.;Phillippe, Mark
早产通常与细胞因子和趋化因子的产生增加有关。这些研究表征了趋化因子单核细胞趋化蛋白-1 （MCP-1）在脂多糖（LPS）诱导的早产小鼠中的表达。在宫内LPS感染后，于妊娠第15天采集CD-1小鼠的子宫和其他组织。定量实时逆转录酶聚合酶链反应检测LPS后24小时内MCP-1和toll样受体4 (TLR4) mRNA的表达。采用细胞因子/趋化因子蛋白阵列、酶联免疫吸附试验和免疫组织化学检测MCP-1蛋白表达。宫内注射LPS导致CD-1小鼠在12 ~ 24小时内早产。MCP-1 rnRNA的表达在2和6小时显著升高，而TLR4的表达在24小时内无显著变化。MCP-1蛋白水平在母体血清、肝、肺、肾和子宫中在2 ~ 6小时达到峰值。免疫组化证实MCP-1存在于子宫肌层和子宫内膜。这些研究提供的证据表明，MCP-1可能在促炎免疫反应中发挥重要作用，导致小鼠早产。
Preterm delivery is often associated with increased cytokine and chemokine production. These studies characterize the expression of the chemokine monocyte chemotactic protein-1 (MCP-1) in mice during lipopolysaccharide (LPS) - induced preterm delivery. Uterine and other tissues were harvested from CD-1 mice on gestational day 15 after intrauterine LPS infection. Quantitative real-time reverse-transcriptase polyrnerase chain reactions determined MCP-1 and toll-like receptor 4 (TLR4) mRNA expression during the 24 hours after LPS. MCP-1 protein expression was determined using a cytokine/chemokine protein array, enzyme-linked immunosorbant assay, and immunohistochemistry. Intrauterine LPS injection caused preterm delivery in CD-1 mice between 12 and 24 hours. Expression of MCP-1 rnRNA significantly increased at 2 and 6 hours, while TLR4 expression did not significantly change over 24 hours. The MCP-1 protein levels peaked by 2 to 6 hours in maternal serum, liver, lung, kidney, and uterus. Immunohistochemistry confirmed MCP-1 in the myometrium and endometrium. These studies provide evidence suggesting that MCP-1 potentially plays an important role during the proinflammatory immune response, leading to preterm labor in the mouse.