Evaluating the efficiency of specimen pooling for PCR-based detection of COVID-19
Evaluating the efficiency of specimen pooling for PCR-based detection of COVID-19
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DOI：
10.1002/jmv.26005
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发表时间：
2020-07-21
影响因子：
12.7
通讯作者：
Hemachudha, Thiravat
中科院分区：
文献类型：
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作者：
Wacharapluesadee, Supaporn;Kaewpom, Thongchai;Hemachudha, Thiravat
在大流行的时代，例如由严重急性呼吸道综合征冠状病毒2（SARS-CoV-2）引起的持续流行，世界面临着检测，个人防护设备的有限供应，工厂和供应链正在努力满足不断增长的需求。本研究旨在评估样本合并用于检测SARS-CoV-2病毒的有效性，以确定是否可以在不影响检测灵敏度的情况下实现成本和资源节约。合并10份先前通过实时聚合酶链反应（PCR）检测的鼻咽和咽拭子标本进行检测，其中包含1份或2份不同病毒浓度的已知阳性标本。当原始标本的PCR循环阈值（Ct）低于35时，标本合并不影响检测SARS-CoV-2的灵敏度。在低病毒载量（Ct > 35）的标本中，15个样本池中有2个（13.3%）为假阴性。汇集样本以检测2019年冠状病毒病低流行率感染（
In the age of a pandemic, such as the ongoing one caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the world faces a limited supply of tests, personal protective equipment, and factories and supply chains are struggling to meet the growing demands. This study aimed to evaluate the efficacy of specimen pooling for testing of SARS-CoV-2 virus, to determine whether costs and resource savings could be achieved without impacting the sensitivity of the testing. Ten previously tested nasopharyngeal and throat swab specimens by real-time polymerase chain reaction (PCR), were pooled for testing, containing either one or two known positive specimens of varying viral concentrations. Specimen pooling did not affect the sensitivity of detecting SARS-CoV-2 when the PCR cycle threshold (Ct) of original specimen was lower than 35. In specimens with low viral load (Ct > 35), 2 of 15 pools (13.3%) were false negative. Pooling specimens to test for Coronavirus Disease 2019 infection in low prevalence (