Nonylphenol enhances apoptosis induced by serum deprivation in PC12 cells.
Nonylphenol enhances apoptosis induced by serum deprivation in PC12 cells.
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DOI：
10.1016/j.lfs.2003.09.066
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发表时间：
2004-03
期刊：
影响因子：
6.1
通讯作者：
M. Aoki;M. Kurasaki;T. Saito;Sayaka Seki;T. Hosokawa;Yasumitsu Takahashi;H. Fujita;T. Iwakuma
中科院分区：
文献类型：
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作者：
M. Aoki;M. Kurasaki;T. Saito;Sayaka Seki;T. Hosokawa;Yasumitsu Takahashi;H. Fujita;T. Iwakuma
尽管壬基酚是众所周知的内分泌干扰物，但有关其生物学效应的信息却很少。本研究采用原位末端标记法和DNA片段分析技术，研究了壬基酚对血清剥夺诱导PC12细胞凋亡的影响。此外，还观察了促凋亡因子Bad、Bax和抗凋亡因子Bcl2含量及caspase 3酶活性的变化。低于100 ng/ml的壬基酚可增加TUNEL信号、DNA片段化和促凋亡因子的含量，与无血清剥夺组比较，差异有统计学意义。此外，加入壬基酚可增强caspase-3的活性，而caspase-3的抑制剂Z-VAD可减弱这种作用。这些结果表明，低于100 ng/ml的壬基酚通过激活caspase-3来增强血清剥夺诱导的PC12细胞的凋亡。
Although nonylphenol is well known as an endocrine disrupting chemical, there is little information concerning biological effect of nonylphenol. In this study, we investigated effect of nonylphenol on apoptosis induced by serum deprivation in PC12 cells using TUNEL and DNA fragmentation assays. In addition, changes in contents of proapoptotic factors, Bad and Bax, and antiapoptotic factor, Bcl-2, and enzyme activity of caspase-3 were studied. Below 100 ng/ml of nonylphenol increased TUNEL signals, DNA fragmentation and content of proapoptotic factor, Bad as compared to those by serum deprivation without nonylphenol. Furthermore, addition of nonylphenol enhanced caspase-3 activity and Z-VAD, caspase-3 inhibitor, diminished such effect. These results indicated that below 100 ng/ml of nonylphenol enhanced apoptosis induced by serum deprivation via caspase-3 activation in PC12 cell.