Aspirin inhibits interleukin 1-induced prostaglandin H synthase expression in cultured endothelial cells.
Aspirin inhibits interleukin 1-induced prostaglandin H synthase expression in cultured endothelial cells.
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阿司匹林抑制培养内皮细胞中白细胞介素 1 诱导的前列腺素 H 合酶表达。
DOI：
10.1073/pnas.88.6.2384
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发表时间：
1991
影响因子：
11.1
通讯作者：
Loose-Mitchell,DS
中科院分区：
文献类型：
--
作者：
Wu,KK;Sanduja,R;Tsai,AL;Ferhanoglu,B;Loose-Mitchell,DS
前列腺素H（PGH）合酶（EC 1.14.99.1）是前列腺素、血栓素和前列环素生物合成中的关键酶。在培养的人脐静脉内皮细胞中，已知白细胞介素1（IL-1）诱导这种酶的合成，从而使PGH合酶蛋白的水平比基础水平提高数倍。用低浓度（0.1-1微克/毫升）的阿司匹林预处理抑制了IL-1诱导的细胞中超过60%的酶质量和环氧合酶活性，而在没有IL-1的细胞中对合成酶的基础水平仅具有最小的影响。水杨酸钠表现出类似的抑制作用，而吲哚美辛没有明显的效果。同样，低水平的阿司匹林抑制了IL-1诱导的L-[35 S]蛋氨酸掺入PGH合酶的增加，也抑制了2.7-蛋氨酸酶PGH合酶mRNA的表达。这些结果表明，在培养的内皮细胞中，阿司匹林或水杨酸盐在PGH合酶基因表达水平上可有效抑制类花生酸生物合成能力。阿司匹林的作用很可能是由于水杨酸盐的降解。
Prostaglandin H (PGH) synthase (EC 1.14.99.1) is a key enzyme in the biosynthesis of prostaglandins, thromboxane, and prostacyclin. In cultured human umbilical vein endothelial cells, interleukin 1 (IL-1) is known to induce the synthesis of this enzyme, thereby raising the level of PGH synthase protein severalfold over the basal level. Pretreatment with aspirin at low concentrations (0.1-1 micrograms/ml) inhibited more than 60% of the enzyme mass and also the cyclooxygenase activity in IL-1-induced cells with only minimal effects on the basal level of the synthase enzyme in cells without IL-1. Sodium salicylate exhibited a similar inhibitory action whereas indomethacin had no apparent effect. Similarly low levels of aspirin inhibited the increased L-[35S]methionine incorporation into PGH synthase that was induced by IL-1 and also suppressed expression of the 2.7-kilobase PGH synthase mRNA. These results suggest that in cultured endothelial cells a potent inhibition of eicosanoid biosynthetic capacity can be effected by aspirin or salicylate at the level of PGH synthase gene expression. The aspirin effect may well be due to degradation of salicylate.
影响因子：
64.8
作者：
HEAVEY, DJ;BARROW, SE;RITTER, JM
通讯作者：
RITTER, JM
用乙酰水杨酸乙酰化前列腺素内过氧化物合成酶。
DOI：
--
发表时间：
1980
期刊：
European Journal of Biochemistry
影响因子：
--
作者：
F. J. van der Ouderaa;M. Buytenhek;D. Nugteren;D. van Dorp
通讯作者：
D. van Dorp