Analysis of a nonribosomal peptide synthetase gene from Alternaria brassicae and flanking genomic sequences
Analysis of a nonribosomal peptide synthetase gene from Alternaria brassicae and flanking genomic sequences
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DOI：
10.1007/s00294-003-0479-z
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发表时间：
2004-04-01
期刊：
影响因子：
2.5
通讯作者：
Simoneau, P
中科院分区：
文献类型：
--
作者：
Guillemette, T;Sellam, A;Simoneau, P
目前关于十字花科植物黑斑病的病原体芸苔链格孢产生的致病决定因素的信息很少。我们筛选了这种真菌的基因组文库，并确定了一个非核糖体肽合成酶（NRPS）基因命名为AbrePsy1。完整的编码序列是22 kbp长，编码一个大的蛋白质（792 kDa），显示典型的NRPS模块组织。AbrePsy1的结构分析揭示了四个完整的延伸模块，其中两个具有差向异构化结构域。在AbrePsy1附近，鉴定了编码ATP结合盒转运蛋白的第二个基因（命名为AbreAtr1）。在宿主植物感染过程中观察到AbrePsy1和AbreAtr1的表达增加。然而，虽然物理上相连，这两个基因可能不是功能性聚类，因为它们的表达模式不同。
Very little information is currently available concerning the pathogenic determinants produced by Alternaria brassicae, the causal agent of the blackspot disease of crucifers. We screened a genomic library of this fungus and identified a nonribosomal peptide synthetase (NRPS) gene named AbrePsy1. The complete coding sequence is 22 kbp long and encodes a large protein (792 kDa) showing typical NRPS modular organization. Structural analysis of AbrePsy1 revealed four complete elongation modules, two of which have epimerization domains. In the vicinity of AbrePsy1, a second gene (named AbreAtr1), which encodes an ATP-binding cassette transporter was identified. Increased expression of AbrePsy1 and AbreAtr1 was observed during host-plant infection. However, while physically linked, these two genes are probably not functionally clustered, as their expression patterns differed.