Microdissection of Mouse Brain into Functionally and Anatomically Different Regions
Microdissection of Mouse Brain into Functionally and Anatomically Different Regions
复制标题
DOI：
10.3791/61941
复制
发表时间：
2021-02-01
影响因子：
1.2
通讯作者：
Gautam, Aarti
中科院分区：
文献类型：
--
作者：
Meyerhoff, James;Muhie, Seid;Gautam, Aarti
大脑是哺乳动物神经系统的指挥中心，是一个结构极其复杂的器官。大脑被保护在头骨内，由一层灰质的外层覆盖在被称为大脑皮层的半球上。在这一层的下面存在着许多其他的专门结构，这些结构对于多种现象的存在至关重要。获取特定大体大脑区域的样本需要快速而精确的解剖步骤。据了解，在微观层面上，存在许多子区域，并可能跨越我们为本解剖目的强加的任意区域边界。小鼠模型通常用于研究人脑功能和疾病。基因表达模式的变化可能局限于特定的大脑区域，根据疾病状态针对特定的表型。因此，从其明确的结构组织来研究转录调控是非常重要的。对大脑的全面了解需要研究不同的大脑区域，定义连接，并确定每个大脑区域活动的关键差异。对这些不同区域的更全面的了解可能会为神经科学领域新的和改进的治疗铺平道路。在这里，我们讨论一步一步的方法解剖小鼠大脑分为16个不同的区域。在这个过程中，我们重点研究了雄性小鼠C57Bl/6J（6-8周龄）的大脑切除和解剖成多个区域，使用神经解剖学标志来识别和采样离散的功能相关和行为相关的大脑区域。这项工作将有助于为神经科学领域奠定坚实的基础，从而在更深入地了解大脑功能方面找到更有针对性的方法。
The brain is the command center for the mammalian nervous system and an organ with enormous structural complexity. Protected within the skull, the brain consists of an outer covering of grey matter over the hemispheres known as the cerebral cortex. Underneath this layer reside many other specialized structures that are essential for multiple phenomenon important for existence. Acquiring samples of specific gross brain regions requires quick and precise dissection steps. It is understood that at the microscopic level, many sub-regions exist and likely cross the arbitrary regional boundaries that we impose for the purpose of this dissection.Mouse models are routinely used to study human brain functions and diseases. Changes in gene expression patterns may be confined to specific brain areas targeting a particular phenotype depending on the diseased state. Thus, it is of great importance to study regulation of transcription with respect to its well-defined structural organization. A complete understanding of the brain requires studying distinct brain regions, defining connections, and identifying key differences in the activities of each of these brain regions. A more comprehensive understanding of each of these distinct regions may pave the way for new and improved treatments in the field of neuroscience. Herein, we discuss a step-by-step methodology for dissecting the mouse brain into sixteen distinct regions. In this procedure, we have focused on male mouse C57Bl/6J (6-8 week old) brain removal and dissection into multiple regions using neuroanatomical landmarks to identify and sample discrete functionally-relevant and behaviorally-relevant brain regions. This work will help lay a strong foundation in the field of neuroscience, leading to more focused approaches in the deeper understanding of brain function.