Long term smoking with age builds up excessive oxidative stress in bronchoalveolar lavage fluid
Long term smoking with age builds up excessive oxidative stress in bronchoalveolar lavage fluid
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DOI：
10.1136/thx.2005.049148
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发表时间：
2006-06-01
期刊：
影响因子：
10
通讯作者：
Nishimura, M.
中科院分区：
文献类型：
--
作者：
Nagai, K.;Betsuyaku, T.;Nishimura, M.
背景：上皮衬里液在保护肺免受氧化应激中起关键作用，氧化状态可能因年龄、吸烟史和肺气肿而改变。方法：对109例有不同吸烟史的青年和老年人进行支气管肺泡灌洗（BAL）。测定BAL液中蛋白羰基、总谷胱甘肽和氧化谷胱甘肽。结果：经Western blot分析，BAL液中主要羰基化蛋白大小为68 kDa，与白蛋白相对应。bald液中每mg总白蛋白中羰基化白蛋白的含量在老年吸烟者中是同龄非吸烟者的4倍，在老年前吸烟者中是3倍（p < 0.0001, p = 0.0003），但在年轻吸烟者中没有。与年龄匹配的非吸烟者相比，年轻吸烟者（p = 0.006）和老年吸烟者（p = 0.0003） BAL液中总谷胱甘肽显著增加。相比之下，与其他组相比，氧化谷胱甘肽与总谷胱甘肽的比例仅在老年吸烟者中显著升高（72%）。这些参数在有轻度肺气肿和没有轻度肺气肿的老年吸烟者之间没有显著差异。结论：氧化谷胱甘肽与过度蛋白羰基化相关，即使在没有肺部疾病的长期吸烟史的老年吸烟者的肺中也会积累，但在轻度肺气肿的吸烟者中没有显著增强。
Background: Epithelial lining fluid plays a critical role in protecting the lung from oxidative stress, in which the oxidised status may change by ageing, smoking history, and pulmonary emphysema.Methods: Bronchoalveolar lavage (BAL) was performed on 109 young and older subjects with various smoking histories. The protein carbonyls, total and oxidised glutathione were examined in BAL fluid.Results: By Western blot analysis, the major carbonylated protein in the BAL fluid was sized at 68 kDa, corresponding to albumin. The amount of carbonylated albumin per mg total albumin in BAL fluid was four times higher in older current smokers and three times higher in older former smokers than in age matched non-smokers (p < 0.0001, p = 0.0003, respectively), but not in young smokers. Total glutathione in BAL fluid was significantly increased both in young (p = 0.006) and older current smokers (p = 0.0003) compared with age matched non-smokers. In contrast, the ratio of oxidised to total glutathione was significantly raised (72%) only in older current smokers compared with the other groups. There was no significant difference in these parameters between older smokers with and without mild emphysema.Conclusions: Oxidised glutathione associated with excessive protein carbonylation accumulates in the lung of older smokers with long term smoking histories even in the absence of lung diseases, but they are not significantly enhanced in smokers with mild emphysema.