Genomes of "phiKMV-like viruses" of Pseudomonas aeruginosa contain localized single-strand interruptions
Genomes of "phiKMV-like viruses" of Pseudomonas aeruginosa contain localized single-strand interruptions
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DOI：
10.1016/j.virol.2009.06.024
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发表时间：
2009-08-15
期刊：
影响因子：
3.7
通讯作者：
Lavigne, Rob
中科院分区：
文献类型：
--
作者：
Kulakov, Leonid A.;Ksenzenko, Vladimir N.;Lavigne, Rob
“phiKV样病毒”包含感染铜绿假单胞菌的T7相关病毒的重要属。这些噬菌体的基因组具有局部单链中断（切口），这是一种独特的基因组特征，以前被认为是T5相关大肠杆菌所独有的。在新测序的噬菌体phi kappa F77中对该特征的分析显示所有四个切口都位于非编码DNA链上。它们以高频率存在于噬菌体群体中，并在裂解周期的后期被引入噬菌体DNA中。切口中的一般共有序列（5 '-CGACxxxxxCCTAoh pCTCCGG-3'）显示在所有phiKV相关的突变中是共同的。(C)2009 Elsevier Inc. All rights reserved.
The "phiKMV-like viruses" comprise an important genus of T7 related phages infecting Pseudomonas aeruginosa. The genomes of these bacteriophages have localized single-strand interruptions (nicks), a distinguishing genomic trait previously thought to be unique for T5 related coliphages. Analysis of this feature in the newly sequenced phage phi kappa F77 shows all four nicks to be localized on the non-coding DNA strand. They are present with high frequencies within the phage Population and are introduced into the phage DNA at late stages of the lytic cycle. The general consensus sequence in the nicks (5'-CGACxxxxxCCTAoh pCTCCGG-3') was shown to be common among all phiKMV-related phages. (C) 2009 Elsevier Inc. All rights reserved.