Laminar segregation of cells of origin of ascending projections from the superficial layers of the superior colliculus in the cat
Laminar segregation of cells of origin of ascending projections from the superficial layers of the superior colliculus in the cat
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猫上丘表层上升投射起源细胞的层状分离
DOI：
10.1016/0006-8993(80)90815-x
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发表时间：
1980
期刊：
影响因子：
2.9
通讯作者：
M. Kudo
中科院分区：
文献类型：
--
作者：
S. Kawamura;N. Fukushima;S. Hattori;M. Kudo
现在人们普遍认为，猫的上丘脑（SC）对几个丘脑结构产生上升投射3,9,12。在这些丘脑结构中，背外侧膝状核（LGNd）和一层后外侧核（Lp的顶盖接受区）据报道接收源自SC浅层的顶盖丘脑纤维3我们已经在猫中使用以下方法进行了证明辣根过氧化物酶（HRP）的逆行运输表明，tecto-Lp投影的起源细胞位于浅灰色层（SC II3）的深处，具有地形排列g，10。在本研究中，我们将证明tecto-geniculate投影的起源细胞与SC II内的tecto-Lp投影的起源细胞不同。对于本研究，进行了两组实验。首先，利用HRP的顺行转运，从层流终止角度确定SC注射HRP后顶盖膝突的终点位置；其次，将 HRP 注射到 LGNd 后，在 SC 中检查逆行标记的细胞，并与 tecto-Lp 投影的细胞进行比较。在3只猫中，将0.2-0.3/zl溶解在含有2,0-二甲基亚砜的无菌0.9%盐水中的50% HRP (Toyobo, Grade 1C)单次显微注射立体定位到左侧SC的中部。在另外 13 只猫中，将 50% HRP 溶液立体定向注射到 LGNd 或 Lp 的顶盖丘脑受体区。存活48-50小时后，在深度麻醉下通过升主动脉向动物灌注2000ml 0.1M磷酸盐缓冲液(pH 7.4)中的1％甲醛和1.25戊二醛的混合物。将整个脑切成适当的额叶块后，取出脑并置于4℃下含有30％蔗糖的相同缓冲液中2-3天。将块在冷冻切片机上以 60# m 切片，然后使用含有联苯胺 2 的孵育介质进行 HRP 处理。在涂胶载玻片上封片后，将切片轻轻擦干。
It is now accepted that the superior colhculus (SC) of the cat gives rise to ascending projections to several thalamic structures3, 9, 12. Of these thalamic structures, the dorsal lateral geniculate nucleus (LGNd) and a tier of the posterior lateral nucleus (tectal recipient zone of Lp) have been reported to receive tecto-thalamic fibers originating in the superficial layers of SC 3 We have already demonstrated in the cat using the method of retrograde transport of horseradish peroxidase (HRP) that cells of origin of the tecto-Lp projection lie in the deep part of the superficial gray layer (SC II3) with a topographical arrangementg, 10. In the present study, we will demonstrate that cells of origin of the tecto-geniculate projection are distinct from those of the tecto-Lp projection within SC II.For the present study, two sets of experiments were carried out. First, by utilizing the anterograde transport of HRP, terminal site of the tecto-geniculate projection was determined after injection of HRP into SC in terms of the laminar termination; and second, retrogradely labeled cells were examined in SC after injection of HRP into LGNd in comparison with those of the tecto-Lp projection. In 3 cats a single microinjection of 0.2-0.3/zl of 50% HRP (Toyobo, Grade lC) dissolved in sterile 0.9% saline containing 2, o dimethylsulfoxide was made stereotaxically into the middle of left SC. In another 13 cats a solution of 50~ HRP was injected stereotaxically into either LGNd or tecto-thalamic recipient zone of Lp. After a survival time of 48-50 h, the animals were perfused with 2000 ml of a mixture of 1% formaldehyde and 1.25 o glutaraldehyde in 0.1 M phosphate buffer (pH 7.4) through the ascending aorta under deep anesthesia. The brains were removed and placed in the same buffer containing 30% sucrose at 4 C for 2-3 days after cutting the whole brain Into appropriate frontal blocks. The blocks were sectioned at 60# m on a freezing microtome and then treated for HRP using an incubation medium containing benzidine 2. After being mounted on gelatinized slides, the sections were lightly