Overexpression of PGC1α and accumulation of p62 in apocrine carcinoma of the breast
Overexpression of PGC1α and accumulation of p62 in apocrine carcinoma of the breast
复制标题
DOI：
10.1111/pin.12235
复制
发表时间：
2015-01-01
影响因子：
2.2
通讯作者：
Masuda, Shinobu
中科院分区：
文献类型：
--
作者：
Fuchinoue, Fumi;Hirotani, Yukari;Masuda, Shinobu
大汗腺癌因其特殊的形态学特征而被归类为一种特殊类型的乳腺癌。为了从线粒体谱的角度阐明大汗腺癌的特征，我们对大汗腺癌和非大汗腺癌进行了比较研究。检测乳腺癌组织中线粒体相关因子PGC1、Nrf1、Nrf2、mtTFA、COX4的表达。大汗腺癌明显倾向于PGC1 mRNA表达水平高于非大汗腺癌。进一步检测所选因子PGC1和p62的表达。结果显示大汗腺癌PGC1免疫组化阳性率较高（21.3% vs. 3.2%, P = 0.008），且大汗腺癌PGC1 mRNA表达水平显著高于非大汗腺癌（P = 0.007）。大泌腺癌中p62蛋白的免疫组化阳性率也较高（44.7%比21.0%,P = 0.015），但两种癌中p62 mRNA的表达水平无显著差异（P = 0.633）。综上所述，本研究揭示了顶泌癌过度表达PGC1有助于线粒体的生物发生，以及p62蛋白的积累。
Apocrine carcinoma is categorized as a special type of breast carcinoma because of its specific morphological features. To clarify the characteristics of apocrine carcinoma from the point of view of the mitochondrial profile, we conducted a comparative study between apocrine and non-apocrine carcinomas. The expressions of mitochondrial related factors (PGC1, Nrf1, Nrf2, mtTFA and COX4) were examined in a testing set of breast cancer tissue. Apocrine carcinomas showed a clear tendency towards higher mRNA expression levels of PGC1 than non-apocrine carcinomas. The expression of the selected factor, PGC1, as well as that of p62 was further examined. The results revealed that apocrine carcinomas showed a higher immunohistochemical positivity rate for PGC1 (21.3% vs. 3.2%; P = 0.008), and that the mRNA expression level of PGC1 was significantly higher in apocrine carcinoma than in non-apocrine carcinoma (P = 0.007). The immunohistochemical positivity rate for p62 protein was also higher in apocrine carcinomas (44.7% vs. 21.0%; P = 0.015), although no significant difference in the p62 mRNA expression level was detected between the two types of carcinoma (P = 0.633). In conclusion, this study revealed that apocrine carcinoma overexpressed PGC1 contributing to mitochondrial biogenesis, and also p62 protein accumulation.