Endophytic root bacteria associated with the natural vegetation growing at the hydrocarbon-contaminated Bitumount Provincial Historic site
Endophytic root bacteria associated with the natural vegetation growing at the hydrocarbon-contaminated Bitumount Provincial Historic site
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DOI：
10.1139/cjm-2017-0039
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发表时间：
2017-06-01
影响因子：
2.8
通讯作者：
Germida, James J.
中科院分区：
文献类型：
--
作者：
Blain, Natalie P.;Helgason, Bobbi L.;Germida, James J.
Bitumount省级历史遗址是世界上最早的两个石油开采和炼油厂的所在地。尽管碳氢化合物水平从330到24700毫克不等。(kg土壤）（-1），植物已经能够通过自然重新植被的方式使该地点恢复生机。本研究的目的是实现更好地了解植物根相关的细菌的伙伴关系发生在自然植被碳氢化合物污染的土壤。根内生细菌群落的特点是从整个网站的代表性植物物种的高通量测序和培养技术。植物种类和采样地点对根际和根内圈细菌种群丰度有显著影响（p < 0.05）。一般来说，放线菌目、根瘤菌目、假单胞菌目、伯克霍尔德菌目和鞘氨醇单胞菌目的成员是最常见的目的。植物种类和取样位置对根际细菌群落结构均有影响。采用实时荧光定量聚合酶链反应技术研究了根内生细菌的功能多样性。16 SrRNA和2个烃降解基因（CYP 153和alkB）的基因拷贝数受植物种类和采样地点的交互作用影响显著（p < 0.05）。我们的研究结果表明，检测到的一些细菌群落已知表现出植物生长促进特性。
The Bitumount Provincial Historic site is the location of 2 of the world's first oil-extracting and -refining operations. Despite hydrocarbon levels ranging from 330 to 24 700 mg.(kg soil)(-1), plants have been able to recolonize the site through means of natural revegetation. This study was designed to achieve a better understanding of the plant-root-associated bacterial partnerships occurring within naturally revegetated hydrocarbon-contaminated soils. Root endophytic bacterial communities were characterized from representative plant species throughout the site by both high-throughput sequencing and culturing techniques. Population abundance of rhizosphere and root endosphere bacteria was significantly influenced (p < 0.05) by plant species and sampling location. In general, members of the Actinomycetales, Rhizobiales, Pseudomonadales, Burkholderiales, and Sphingomonadales orders were the most commonly identified orders. Community structure of root-associated bacteria was influenced by both plant species and sampling location. Quantitative real-time polymerase chain reaction was used to determine the potential functional diversity of the root endophytic bacteria. The gene copy numbers of 16S rRNA and 2 hydrocarbon-degrading genes (CYP153 and alkB) were significantly affected (p < 0.05) by the interaction of plant species and sampling location. Our findings suggest that some of the bacterial communities detected are known to exhibit plant growth promotion characteristics.